Cytokine gene expression profile distinguishes CD4+/CD57+T cells of the nodular lymphocyte predominance type of Hodgkin's lymphoma from their tonsillar counterparts

Autor: Lydia Visser, Sibrand Poppema, Anke van den Berg, Cigdem Atayar
Přispěvatelé: Faculteit Medische Wetenschappen/UMCG, Stem Cell Aging Leukemia and Lymphoma (SALL), Translational Immunology Groningen (TRIGR)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
CD4-Positive T-Lymphocytes
Male
Pathology
nodular lymphocyte predominance type of Hodgkin's lymphoma
Lymphocyte
T-Lymphocytes
Palatine Tonsil
DISEASE
CD57 Antigens
Progressive transformation of germinal centres
INDUCED CYTIDINE DEAMINASE
PROGRESSIVE TRANSFORMATION
Oligonucleotide Array Sequence Analysis
Interleukin-13
Hodgkin's lymphoma
IMMUNE-RESPONSES
Middle Aged
Flow Cytometry
Hodgkin Disease
progressive transformation of germinal centres
medicine.anatomical_structure
B-CELLS
Female
Adult
medicine.medical_specialty
Adolescent
T cell
T cells
Biology
Pathology and Forensic Medicine
Interferon-gamma
medicine
Humans
REED-STERNBERG CELLS
Interleukin 4
Aged
Hyperplasia
Gene Expression Profiling
Germinal center
BCL-6 PROTEIN
T lymphocyte
medicine.disease
Molecular biology
cytokines
Reed–Sternberg cell
GERMINAL-CENTERS
T-CELLS
Interleukin-2
Interleukin-4
INTERLEUKIN-13 RECEPTOR
CD4(+)/CD57(+) T cells
Zdroj: JOURNAL OF PATHOLOGY, 208(3), 423-430. Wiley
ISSN: 0022-3417
DOI: 10.1002/path.1894
Popis: Little is known about the cytokine profile of nodular lymphocyte predominance Hodgkin's lymphoma (NLPHL) and the significance of the characteristic rosetting CD4(+)/CD57(+) T cells. We analysed the T lymphocyte populations isolated from lymph node suspensions from five patients with NLPHL, two with follicular hyperplasia and progressive transformation of germinal centres (PTGC), three with classical Hodgkin's lymphoma (CHL) and five with hyperplasia of the tonsil. We sorted the T cells based on expression of CD3, CD4 and CD57 by flow cytometry and evaluated the cytokine mRNA profiles of the T cells with quantitative RT-PCR. NLPHL cases were as rich in T cells as the CHL cases, but all NLPHL cases had a much higher frequency of CD4(+)/CD57(+) T cells. In contrast to the CD4(+)/CD57(+) T cells from tonsils, IL2 and IL4 mRNAs were consistently absent from the CD4(+)/CD57(+) T cells of NLPHL. Even after stimulation, no IL4 transcripts could be detected in the CD4(+)/CD57(+) T cells of NLPHL. On the other hand, IFN gamma transcripts were elevated in NLPHL and PTGC T cell subsets as compared to tonsillar T cell subsets. IL13 mRNA was exclusively produced by the T cells of CHL cases, indicating that IL13 may be a key cytokine in CHL. In conclusion, elevated levels of CD4(+)/CD57(+) T cells are characteristic of NLPHL and these T cells display a distinct cytokine mRNA profile. Copyright (c) 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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