Screening for alkaline keratinolytic activity in fungi isolated from soils of the biosphere reserve 'Parque Costero del Sur' (Argentina)
| Autor: | Lorena Alejandra Elíades, Mario Carlos Nazareno Saparrat, Claudio Enrique Voget, Marta Noemí Cabello, Betina Galarza |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Physiology
Soybean meal Argentina Cladosporium cladosporioides Applied Microbiology and Biotechnology Alkali soil chemistry.chemical_compound Soil Soil pH Botany Ciencias Naturales Food science Parque Costero del Sur Ciencias Exactas Biosphere reserve biology Aspergillus niger Fungi food and beverages General Medicine biology.organism_classification Keratinase chemistry Solid-state fermentation Alkaline keratinases Tryptone biology.protein Biotechnology |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| Popis: | A screening was carried out on 69 fungal strains isolated from alkaline-calcareous, neutral and alkaline-sodic soils, as well as from their associated plant material, to determine their ability to grow at alkaline pH. A total of 32 fungi were selected for their ability to produce alkaline keratinase activity in submerged shaken cultures supplemented with soybean meal (SM) and tryptone and on cow hair (CH) under solid state fermentation conditions. Although several fungal strains produced keratinolytic activity on both SM and CH, they differed in the levels detected. Among them, Aspergillus niger, Cladosporium cladosporioides, Metarrhizium anisopliae, Neurospora tetrasperma and Westerdikella dispersa were the best producers, with levels higher than 1.2 U ml⁻¹. Different fungal species are here reported for the first time for their ability to produce keratinolytic activity at alkaline pH. Instituto de Botánica "Dr. Carlos Spegazzini" Centro de Investigación y Desarrollo en Fermentaciones Industriales Instituto de Fisiología Vegetal |
| Databáze: | OpenAIRE |
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