Agrobacterium-Mediated Transient Transformation of Marchantia Liverworts

Autor:	Hidekazu Iwakawa, Hiroyasu Motose, Katharina Melkonian, Hyung-Woo Jeon, Ryuichi Nishihama, Titus Schlüter, Hirofumi Nakagami
Rok vydání:	2021
Předmět:	biology Physiology Agrobacterium fungi Marchantia food and beverages Cell Biology Plant Science General Medicine Agrobacterium tumefaciens biology.organism_classification Plants Genetically Modified Cell biology Marchantia polymorpha Transformation (genetics) Pathosystem Rhizoid Transformation Genetic Transduction Genetic Gene expression
Zdroj:	Plant and Cell Physiology
ISSN:	1471-9053
Popis:	Agrobacterium-mediated transient gene expression is a rapid and useful approach for characterizing functions of gene products in planta. However, the practicability of the method in the model liverwort Marchantia polymorpha has not yet been thoroughly described. Here we report a simple and robust method for Agrobacterium-mediated transient transformation of Marchantia thalli and its applicability. When thalli of M. polymorpha were co-cultured with Agrobacterium tumefaciens carrying β-glucuronidase (GUS) genes, GUS staining was observed primarily in assimilatory filaments and rhizoids. GUS activity was detected 2 days after infection and saturated 3 days after infection. We were able to transiently co-express fluorescently tagged proteins with proper localizations. Furthermore, we demonstrate that our method can be used as a novel pathosystem to study liverwort–bacteria interactions. We also provide evidence that air chambers support bacterial colonization.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e5d7b95031c757ddb4c0e38d48b6e20 https://pubmed.ncbi.nlm.nih.gov/34383076 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.