Acyclic Triterpenoid Isolated from Alpinia katsumadai Alleviates Formalin-Induced Chronic Mouse Paw Inflammation by Inhibiting the Phosphorylation of ERK and NF-κB
| Autor: | Seung Woong Lee, Hee Ju Lim, Sang-Ik Park, Seon Gyeong Bak, Seung-Jae Lee, Soyoung Lee, Mun-Chual Rho, Hyung Jin Lim, Sae-Kwang Ku |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
MAPK/ERK pathway
Lipopolysaccharide Pharmaceutical Science Inflammation Pharmacology Analytical Chemistry Proinflammatory cytokine lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry Drug Discovery medicine Extracellular Physical and Theoretical Chemistry chronic mouse model 030304 developmental biology 0303 health sciences Alpinia katsumadai biology Kinase Organic Chemistry NF-κB anti-inflammation 2 3 5 22 23-pentahydroxy-2 6 10 15 19 23-hexamethyl-tetracosa-6 10 14 18-tetraene chemistry Chemistry (miscellaneous) 030220 oncology & carcinogenesis biology.protein Molecular Medicine Cyclooxygenase medicine.symptom |
| Zdroj: | Molecules, Vol 25, Iss 3345, p 3345 (2020) Molecules Volume 25 Issue 15 |
| ISSN: | 1420-3049 |
| Popis: | Chronic and excessive inflammation can destroy host organs and cause inflammatory diseases such as inflammatory bowel disease, asthma, and rheumatoid arthritis. In this study, we investigated the anti-inflammatory effects of Alpinia katsumadai seed-derived 2,3,5,22,23-pentahydroxy-2,6,10,15,19,23-hexamethyl-tetracosa-6,10,14,18-tetraene (PHT) using lipopolysaccharide (LPS)-stimulated J774 cells and a formalin-induced chronic paw inflammation mouse model. The in vitro results showed that PHT exhibited no cytotoxicity and decreased LPS-induced NO secretion. Additionally, PHT inhibited LPS-induced inducible NO synthase (iNOS) and cyclooxygenase 2 (COX2) protein expression. The quantitative real-time PCR results showed that PHT downregulated the gene expression of the proinflammatory cytokines interleukin-1&beta (IL-1&beta ) and interleukin-6 (IL-6) but not tumor necrosis factor &alpha (TNF-&alpha ). PHT inhibited the LPS-induced phosphorylation of extracellular signal-regulated kinase (ERK) and nuclear factor kappa light chain enhancer of activated B cells (NF-&kappa B). In a mouse model, oral administration of 50 mg/kg PHT significantly alleviated both mouse paw thickness and volume. These results indicate that PHT has potential anti-inflammatory effects and should be considered a possible functional material. |
| Databáze: | OpenAIRE |
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