A cytoplasmic gene for partial suppression of a nuclear pleiotropic respiratory deficient mutant in the petite negative yeast Schizosaccharomyces pombe
Autor: | F Labaille, Anne-Marie Colson, André Goffeau |
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Rok vydání: | 1976 |
Předmět: |
Adenosine Triphosphatases
Mutant Extrachromosomal Inheritance Mutagenesis (molecular biology technique) Biology biology.organism_classification Phenotype Molecular biology law.invention chemistry.chemical_compound Suppression Genetic Ascomycota chemistry law Mutation Schizosaccharomyces Schizosaccharomyces pombe Genetics Cytochromes Suppressor Ethidium bromide Molecular Biology Gene Mitosis |
Zdroj: | Molecular and General Genetics MGG. 149:101-109 |
ISSN: | 1432-1874 0026-8925 |
Popis: | The nuclear pleiotropic respiratory-deficient mutant pet1 (previously M126) exhibits cytochromes aa3 and b deficiencies accompanied by loss of the oligomycin-sensitivity of the mitochondrial ATPase. The mutant pet1, unable to grow on glycerol, growth on glucose. The latter phenotypic trait symbolized by ANAS-D, exhibits a high frequency (2 to 4 X 10(5)) Of spontaneous suppression into Antimycin A-resistant strains. Mutagenesis with MnCl2 increases by a factor of 10(2) the frequency of ANAR-D derivatives. This suppression is partial since none of the suppressed strains is able to grow on glycerol even when respiratory functions and cytochromes activities are restored as in the pet1 [SUP2] strain. In the latter strain it is concluded that the extralocus suppressor gene [SUP2] is responsible for the ANAR-D trait. Tetrad analysis in a cross homozygous for pet1 demonstrates a non-Mendelian segregation pattern for the SUP2 suppressor gene. In stable diploids, homozygous for pet1, the [SUP2] suppressor exhibits a mitotic segregation pattern. Furthermore the transmission of the [SUP2] gene is decreased by ethidium bromide treatment. Therefore, the [SUP2] suppressor gene responsible for partial suppression of the nuclear pleiotropic phenotype in mutant pet1 is of cytoplasmic heredity. |
Databáze: | OpenAIRE |
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