Comparison of nucleotide sequences among hantaviruses belonging to the same serotype: an analysis of amplified DNA by thermal cycle sequencing
| Autor: | Pilaipan Puthavathana, Ho Wang Lee, Luck-Ju Baek, M. Dobbs, C. Y. Kang, Yong-Kyu Chu |
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| Rok vydání: | 1993 |
| Předmět: |
Serotype
Orthohantavirus Cancer Research Molecular Sequence Data Polymerase Chain Reaction Homology (biology) law.invention Viral Proteins chemistry.chemical_compound law Sequence Homology Nucleic Acid Virology Amino Acid Sequence Typing Serotyping Polymerase chain reaction Hantavirus Genetics Base Sequence Sequence Homology Amino Acid biology Nucleic acid sequence virus diseases Sequence Analysis DNA biology.organism_classification Infectious Diseases chemistry DNA Viral Bunyaviridae DNA |
| Zdroj: | Virus Research. 30:161-169 |
| ISSN: | 0168-1702 |
| Popis: | The hantavirus genus, belonging to the bunyaviridae family, is comprised of at least four serologically distinct types: Hantaan, Seoul, Puumala and Prospect Hill. Previously, we reported the use of the polymerase chain reaction (PCR) for grouping hantavirus isolates by using four sets of primers specific to each serotype. Our PCR typing results agreed with those of serological typing. The present study makes use of thermal cycle sequencing to sequence PCR-amplified DNA products in order to determine the level of similarity among members of the same serotype. We show that members of Hantaan and Seoul serotypes are over 92% homologous, irrespective of their host and geographical origin. Puumala sequences show a degree of homology ranging from 80 to 98%. Despite the variation in sequence at the nucleotide level, amino acids show an even higher level of conservation. |
| Databáze: | OpenAIRE |
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