Atypical Myxomatosis - Virus Isolation, Experimental Infection of Rabbits and Restriction Endonuclease Analysis of the Isolate
| Autor: | B. Šmíd, J. Bendová, I. Psikal, L. Valíček, L. Rodák |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Myxomatosis
biology Strain (biology) Myxoma virus Virulence Enzyme-Linked Immunosorbent Assay Viral Vaccines General Medicine Antibodies Viral biology.organism_classification medicine.disease Virology Vaccination Restriction enzyme genomic DNA Myxomatosis Infectious DNA Viral medicine Animals Rabbits Restriction fragment length polymorphism Polymorphism Restriction Fragment Length Czech Republic |
| Zdroj: | Journal of Veterinary Medicine Series B. 50:259-264 |
| ISSN: | 1439-0450 0931-1793 |
| DOI: | 10.1046/j.1439-0450.2003.00665.x |
| Popis: | Atypical form of myxomatosis, which caused non-lethal and clinically mild disease in domestic rabbits 1 month after immunization with a commercially available vaccine MXT, is described. The isolated myxoma virus designated as Litovel 2 (Li-2) did not induce systemic disease following subcutaneous and intradermal applications in susceptible experimental rabbits but led to the immune response demonstrated by ELISA. No severe disease was induced in those Li-2 inoculated rabbits by challenge with the virulent strains Lausanne (Lu) or Sanar (SA), while the control animals showed nodular form of myxomatosis with lethal course of the illness. Restriction fragment length polymorphism (RFLP) of genomic DNA with KpnI and BamHI endonucleases was used for genetic characterization of the Li-2 isolate, the vaccine strain MXT and both virulent strains Lu and SA, respectively. In general, RFLP analysis has shown to be informative for inferring genetic relatedness between myxoma viruses. Based on restriction endonuclease DNA fragment size distribution, it was evident that the pathogenic strain SA is genetically related to the reference strain Lu and the isolate Li-2 is more related, but not identical, to the vaccination strain MXT. |
| Databáze: | OpenAIRE |
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