The Transcriptional Response to Androgens of the Rat VCSA1 Gene Is Amplified by Both Binary and Graded Mechanisms
| Autor: | Catherine Rougeot, François Rougeon, Isabelle Rosinski-Chupin, Jean-François Huaulmé |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Transcription Genetic Submandibular Gland Cell Population In situ hybridization Biology Endocrinology Pregnancy Internal medicine Gene expression medicine Animals Protein Precursors Rats Wistar Salivary Proteins and Peptides education Gene In Situ Hybridization Regulation of gene expression Messenger RNA education.field_of_study Molecular biology Submandibular gland Rats medicine.anatomical_structure Gene Expression Regulation Androgens Female |
| Zdroj: | Endocrinology. 142:4550-4559 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.142.10.8428 |
| Popis: | In higher eukaryotes, gene expression can be highly modified in response to small variations of circulating hormonal inducers. To determine the mechanisms responsible for the 100- to 200-fold enhancement of expression of an androgen-regulated gene, VCSA1, in the acinar cells of rat submandibular glands during puberty, we performed a detailed analysis of VCSA1 expression at the single cell level. Using in situ detection of mature and primary VCSA1 transcripts, we show that VCSA1 expression is activated in only a small proportion of differentiated acinar cells in the presence of low levels of circulating androgens in prepubescent and in castrated males, as well as in females. During the time course of sexual maturation in males, we demonstrate an increase in the proportion of acinar cells expressing VCSA1 and an increase in VCSA1 heterogeneous nuclear RNA and mRNA content in the positive cell population. Finally, we show that changes in the methylation pattern of VCSA1 are correlated with VCSA1 transcriptional activation. These results demonstrate that androgens can, in physiological conditions, elicit both a binary and a graded response. They also provide evidence that the range of gene regulation may be expanded by a transcriptional repression in a majority of cells under basal conditions. |
| Databáze: | OpenAIRE |
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