Cytokine-dependent anti-viral role of CD4-positive T cells in therapeutic vaccination against chronic hepatitis B viral infection
| Autor: | Kiwamu Okita, Yuhki Yamaguchi, Hiroaki Ishiko, Keiko Korenaga, Keisuke Hino, Takahiro Yamasaki, Akio Hayashi, Kiyomi Funatsuki, Fenyu Ren, Muneko Furutani, Tomomi Konishi, Satoyoshi Yamashita |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Hepatitis B virus medicine.medical_treatment T cell Lymphocyte Activation medicine.disease_cause Interferon-gamma Hepatitis B Chronic Antigen Virology Humans Cytotoxic T cell Medicine Hepatitis B Vaccines Prospective Studies Aged Hepatitis B Surface Antigens Tumor Necrosis Factor-alpha business.industry ELISPOT Immunotherapy Active Middle Aged Vaccination Treatment Outcome Infectious Diseases Cytokine medicine.anatomical_structure Immunology Cytokines Female business CD8 |
| Zdroj: | Journal of Medical Virology. 71:376-384 |
| ISSN: | 1096-9071 0146-6615 |
| Popis: | There are several lines of evidence suggesting that specific vaccine therapy with a standard hepatitis B virus (HBV) vaccination reduces HBV replication. The aim of this study was to investigate the anti-viral mechanism of vaccine therapy in chronic hepatitis B patients. Nineteen patients were assigned to receive either vaccine therapy (n = 13) or no treatment as a control (n = 6). Vaccinated patients were analyzed for T cell proliferative responses specific for envelope antigen and cytokine production by antigen-specific T cells. ELISPOT and cytotoxicity assays also were carried out for limited blood samples. Serum HBV DNA levels decreased significantly at 3 months after completion of therapy and thereafter as compared to the baseline ones, and were significantly lower in vaccinated patients than in controls at 12 and 18 months after completion of therapy. Vaccination induced antigen-specific CD4+ T cell proliferative responses in four patients (30.8%). The production of high levels of interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) by antigen-specific T cells was found in six patients (46.0%) who showed significantly lower HBV DNA levels in serum at 6 (P = 0.04) and 18 months (P = 0.005) after completion of therapy than those without high levels of cytokine production. Vaccination did not induce antigen-specific CD8+ T cells or cytotoxic T cells. These results suggest that envelope-specific CD4+ T cells may control directly HBV replication by producing anti-viral cytokines rather than providing help for cytotoxic T cells in therapeutic vaccination against chronic HBV infection. J. Med. Virol. 71:376–384, 2003. © 2003 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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