Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence
Autor: | Yurong Xie, Mengdi Ma, Yang Liu, Baobao Wang, Hongbin Wei, Dexin Kong, Haiyang Wang |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Senescence
EIN3 biology Phytochrome FHY3/FAR1 leaf senescence fungi PIF5 Arabidopsis Plant culture food and beverages Plant Science biology.organism_classification Cell biology SB1-1110 ORE1 chemistry.chemical_compound chemistry Plant hormone Psychological repression Transcription factor DNA Function (biology) Original Research |
Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Vol 12 (2021) |
ISSN: | 1664-462X |
Popis: | Leaf senescence is the terminal stage of leaf development. Both light and the plant hormone ethylene play important roles in regulating leaf senescence. However, how they coordinately regulate leaf senescence during leaf development remains largely unclear. In this study, we show that FHY3 and FAR1, two homologous proteins essential for phytochrome A-mediated light signaling, physically interact with and repress the DNA binding activity of EIN3 (a key transcription factor essential for ethylene signaling) and PIF5 (a bHLH transcription factor negatively regulating light signaling), and interfere with their DNA binding to the promoter of ORE1, which encodes a key NAC transcription factor promoting leaf senescence. In addition, we show that FHY3, PIF5, and EIN3 form a tri-protein complex(es) and that they coordinately regulate the progression of leaf senescence. We show that during aging or under dark conditions, accumulation of FHY3 protein decreases, thus lifting its repression on DNA binding of EIN3 and PIF5, leading to the increase of ORE1 expression and onset of leaf senescence. Our combined results suggest that FHY3 and FAR1 act in an age gating mechanism to prevent precocious leaf senescence by integrating light and ethylene signaling with developmental aging. |
Databáze: | OpenAIRE |
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