Tumor-Derived Heat Shock Protein 70 Peptide Complexes Are Cross-Presented by Human Dendritic Cells
Autor: | Anna Brandl, Rolf D. Issels, Elisabeth Kremmer, Robert Gastpar, Valeria Milani, Stuart K. Calderwood, Alexzander Asea, Elfriede Noessner, Peter Hutzler, Miriam Roos, Maria C. Kuppner |
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Rok vydání: | 2002 |
Předmět: |
Macromolecular Substances
T-Lymphocytes medicine.medical_treatment T cell Immunology Tyrosinase Peptide Biology Lymphocyte Activation Monocytes Antigens Neoplasm Heat shock protein MHC class I Tumor Cells Cultured medicine Humans Immunology and Allergy Cytotoxic T cell HSP70 Heat-Shock Proteins Receptors Immunologic Melanoma Cell Line Transformed Antigen Presentation Monophenol Monooxygenase Cell Membrane Histocompatibility Antigens Class I Cell Differentiation Dendritic Cells Immunotherapy Molecular biology Clone Cells Hsp70 Protein Transport medicine.anatomical_structure biology.protein Clone (B-cell biology) Oligopeptides Protein Binding Signal Transduction |
Zdroj: | Europe PubMed Central Scopus-Elsevier |
ISSN: | 1550-6606 0022-1767 |
Popis: | Our study demonstrates that tumor-derived heat shock protein (HSP)70 chaperones a tyrosinase peptide and mediates its transfer to human immature dendritic cells (DCs) by receptor-dependent uptake. Human tumor-derived HSP70 peptide complexes (HSP70-PC) thus have the immunogenic potential to instruct DCs to cross-present endogenously expressed, nonmutated, and tumor antigenic peptides that are shared among tumors of the melanocytic lineage for T cell recognition. T cell stimulation by HSP70-instructed DCs is dependent on the Ag bound to HSP70 in that only DCs incubated with HSP70-PC purified from tyrosinase-positive (HSP70-PC/tyr+) but not from tyrosinase-negative (HSP70-PC/tyr−) melanoma cells resulted in the specific activation of the HLA-A*0201-restricted tyrosinase peptide-specific cytotoxic T cell clone. HSP70-PC-mediated T cell stimulation is very efficient, delivering the tyrosinase peptide at concentrations as low as 30 ng/ml of HSP70-PC for T cell recognition. Receptor-dependent binding of HSP70-PC and active cell metabolism are prerequisites for MHC class I-restricted cross-presentation and T cell stimulation. T cell stimulation does not require external DC maturation signals (e.g., exogenously added TNF-α), suggesting that signaling DC maturation is an intrinsic property of the HSP70-PC itself and related to receptor-mediated binding. The cross-presentation of a shared human tumor Ag together with the exquisite efficacy are important new aspects for HSP70-based immunotherapy in clinical anti-cancer vaccination strategies, and suggest a potential extension of HSP70-based vaccination protocols from a patient-individual treatment modality to its use in an allogeneic setting. |
Databáze: | OpenAIRE |
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