Aptamer based point of care diagnostic for the detection of food allergens
| Autor: | David Fleischer, Vasant Chellappa, Olivia Alley, Adi Gilboa-Geffen, Sarah Stidham, Wayne G. Shreffler, Hugh A. Sampson, Jonathan M. Spergel, Lucas Yoder, Valerie Villareal |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Arachis
Aptamer Science Sensitivity and Specificity Article Medicine Humans Food allergens Point of care Plant Proteins Multidisciplinary business.industry Assay systems Membrane Proteins food and beverages DNA Allergens Antigens Plant Aptamers Nucleotide Point-of-Care Testing Immunology business Food Analysis Food Hypersensitivity Protein Binding |
| Zdroj: | Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Aptamers, due to their small size, strong target affinity, and ease of chemical modification, are ideally suited for molecular detection technologies. Here, we describe successful use of aptamer technology in a consumer device for the detection of peanut antigen in food. The novel aptamer-based protein detection method is robust across a wide variety of food matrices and sensitive to peanut protein at concentrations as low as 12.5 ppm (37.5 µg peanut protein in the sample). Integration of the assay into a sensitive, stable, and consumer friendly portable device will empower users to easily and quickly assess the presence of peanut allergens in foods before eating. With most food reactions occurring outside the home, the type of technology described here has significant potential to improve lives for children and families. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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