Fucoxanthin Holds Potential to Become a Drug Adjuvant in Breast Cancer Treatment: Evidence from 2D and 3D Cell Cultures

Autor:	Carla Costa, Alice A. Ramos, Fernanda Malhão, Eduardo Rocha, Ana Catarina Macedo
Přispěvatelé:	Instituto de Saúde Pública da Universidade do Porto
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	0301 basic medicine combinatorial therapy Cell Culture Techniques Pharmaceutical Science cisplatin Antineoplastic Agents Breast Neoplasms Xanthophylls doxorubicin Article Analytical Chemistry fucoxanthin 03 medical and health sciences chemistry.chemical_compound QD241-441 0302 clinical medicine Cell Line Tumor Drug Discovery medicine Humans Fucoxanthin Cytotoxic T cell Doxorubicin Physical and Theoretical Chemistry Cytotoxicity Adjuvants Pharmaceutic Cell Proliferation Cisplatin Cell Death Cell growth Organic Chemistry Drug Synergism 030104 developmental biology chemistry Chemistry (miscellaneous) SKBR3 Apoptosis 030220 oncology & carcinogenesis Cancer research triple-negative breast cancer Molecular Medicine Female medicine.drug seaweed compounds
Zdroj:	Molecules Volume 26 Issue 14 Molecules, Vol 26, Iss 4288, p 4288 (2021)
ISSN:	1420-3049
DOI:	10.3390/molecules26144288
Popis:	(This article belongs to the Special Issue Compounds from Marine Sources as Hits and Leads for Pharmaceutical, Cosmeceutical and Industrial Applications) Fucoxanthin (Fx) is a carotenoid derived from marine organisms that exhibits anticancer activities. However, its role as a potential drug adjuvant in breast cancer (BC) treatment is still poorly explored. Firstly, this study investigated the cytotoxic effects of Fx alone and combined with doxorubicin (Dox) and cisplatin (Cis) on a panel of 2D-cultured BC cell lines (MCF7, SKBR3 and MDA-MB-231) and one non-tumoral cell line (MCF12A). Fucoxanthin induced cytotoxicity against all the cell lines and potentiated Dox cytotoxic effects towards the SKBR3 and MDA-MB-231 cells. The combination triggering the highest cytotoxicity (Fx 10 µM + Dox 1 µM in MDA-MB-231) additionally showed significant induction of cell death and genotoxic effects, relative to control. In sequence, the same combination was tested on 3D cultures using a multi-endpoint approach involving bioactivity assays and microscopy techniques. Similar to 2D cultures, the combination of Fx and Dox showed higher cytotoxic effects on 3D cultures compared to the isolated compounds. Furthermore, this combination increased the number of apoptotic cells, decreased cell proliferation, and caused structural and ultrastructural damages on the 3D models. Overall, our findings suggest Fx has potential to become an adjuvant for Dox chemotherapy regimens in BC treatment. The Strategic Funding UIDB/04423/2020 and UIDP/04423/2020 partially supported this research, through national funds provided by FCT and ERDF to CIIMAR/CIMAR, in the framework of the program PT2020. The Doctoral Program in Biomedical Sciences, of the ICBAS-University of Porto, offered additional funds. info:eu-repo/semantics/publishedVersion
Databáze:	OpenAIRE
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