Studies on rat and human thymus to demonstrate immunoreactivity of calcitonin gene-related peptide, tyrosine hydroxylase and neuropeptide Y
| Autor: | Andrea Kranz, Brita von Gaudecker, Marion D. Kendall |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Aging Histology Tyrosine 3-Monooxygenase Calcitonin Gene-Related Peptide Thymus Gland Calcitonin gene-related peptide Biology Paracrine signalling Dopamine Internal medicine Cortex (anatomy) medicine Animals Humans Neuropeptide Y Child Microscopy Immunoelectron Molecular Biology Ecology Evolution Behavior and Systematics Medulla Tyrosine hydroxylase Infant Epithelial Cells Cell Biology Neuropeptide Y receptor Immunohistochemistry Rats medicine.anatomical_structure Endocrinology Animals Newborn Calcitonin Rats Inbred Lew Child Preschool Female Anatomy Developmental Biology medicine.drug Research Article |
| Zdroj: | Journal of anatomy. 191 |
| ISSN: | 0021-8782 |
| Popis: | The peptidergic and noradrenergic innervation of rat and human thymus was investigated by immunohistochemistry at the light and electron microscopical level (avidin-biotin-complex, sucrose-phosphate-glyoxylic-acid, and immunogold techniques). The distribution of noradrenergic neural profiles, and positive immunoreactivity for calcitonin gene-related peptide (CGRP), tyrosine hydroxylase (TH) and neuropeptide Y (NPY) is described in female rats during ageing, and in human children. In the neonatal rat thymus, the arteries and septa are well supplied by fine varicose nerves. In older animals (2 wk–1 y) the number of septa and blood vessels increase and consequently also the innervation. No nerves were found in the cortex. Apart from the innervation of the septal areas, immunoreactivity for CGRP and TH was present in thymic cells. Except for the young rats (neonatal–14 d), all rats showed CGRP positivity in subcapsular/perivascular epithelial cells (type 1 cells). All rat thymuses also contained a few TH positive cells in the medulla, which could only be confirmed as epithelial cells (type 6 cells) in children. Type 1 cells in the human thymus were not CGRP positive, but as in the rat, there were similar TH positive cells in the medulla. It was concluded that in addition to nerves containing CGRP, noradrenaline or dopamine, epithelial cells also contain these transmitters. They could therefore act on different cells (compared with neural targets) in a paracrine manner. |
| Databáze: | OpenAIRE |
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