Anandamide Increases the Differentiation of Rat Adipocytes and Causes PPARγ and CB1 Receptor Upregulation

Autor: Mary Mavri-Vavayanni, Athanasia Siafaka-Kapadai, Katerina Psarra, Chrisanthi Gontinou, Sevasti Karaliota
Rok vydání: 2009
Předmět:
Male
medicine.medical_specialty
Cannabinoid receptor
Polyunsaturated Alkamides
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Cellular differentiation
Blotting
Western
Indomethacin
Medicine (miscellaneous)
Peroxisome proliferator-activated receptor
Arachidonic Acids
Amidohydrolases
chemistry.chemical_compound
Endocrinology
Receptor
Cannabinoid
CB1
Internal medicine
Adipocyte
Adipocytes
medicine
Animals
Enzyme Inhibitors
Rats
Wistar
chemistry.chemical_classification
Nutrition and Dietetics
Reverse Transcriptase Polymerase Chain Reaction
Chemistry
Cell Differentiation
Anandamide
URB597
Flow Cytometry
Endocannabinoid system
Rats
Up-Regulation
PPAR gamma
Adipose Tissue
Cyclooxygenase 2
Benzamides
lipids (amino acids
peptides
and proteins)
Carbamates
Cannabinoid
Endocannabinoids
Zdroj: Obesity. 17:1830-1838
ISSN: 1930-7381
DOI: 10.1038/oby.2009.177
Popis: Anandamide (N-arachidonoylethanolamine, AEA) or its metabolites participate in energy balance mainly through feeding modulation. In addition, AEA has been found to increase 3T3-L1 adipocyte differentiation process. In this study, the effect of AEA, R(+)-methanandamide (R(+)-mAEA), URB597, and indomethacin on primary rat adipocyte differentiation was evaluated by a flow cytometry method and by Oil Red-O staining. Reverse transcription-PCR and western blotting analysis were performed in order to study the effect of AEA on peroxisome proliferator-activated receptor (PPAR)gamma2, cannabinoid receptors (CBRs), fatty acid amidohydrolase (FAAH), and cyclooxygenase-2 (COX-2) expression, during the differentiation process. AEA increased adipocyte differentiation in primary cell cultures in a concentration- and time-dependent manner and induced PPARgamma2 gene expression, confirming findings with 3T3-L1 cell line. CB1R, FAAH, and COX-2 expression was also increased while CB2R expression was decreased. Inhibition of FAAH and COX-2 attenuated the AEA-induced differentiation. Our findings indicate that AEA regulates energy homeostasis not only by appetite modulation but may also regulate adipocyte differentiation and phenotype.
Databáze: OpenAIRE
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