The human growth hormone (hGH) antagonist G120RhGH does not antagonize GH in the rat, but has paradoxical agonist activity, probably via the prolactin receptor
| Autor: | I. C. A. F. Robinson, W. Y. Chen, J. J. Kopchick, Timothy N. C. Wells, R. G. Clark, P. Tollet, D. F. Carmignac, Agneta Mode |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Agonist endocrine system medicine.medical_specialty Receptors Prolactin medicine.drug_class Liver cytology Injections Subcutaneous Dwarfism Biology Rats Sprague-Dawley Endocrinology In vivo Internal medicine medicine Animals Humans Receptor Cells Cultured Hypophysectomy Prolactin receptor Homozygote Antagonist Recombinant Proteins Growth hormone secretion Prolactin Rats Somatropin Liver Growth Hormone Injections Intravenous hormones hormone substitutes and hormone antagonists |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.137.2.8593788 |
| Popis: | Human GH (hGH) acts by dimerizing two hGH receptors that bind to different sites in hGH. G120RhGH, an analog mutated in the second binding site to prevent receptor dimerization, acts as an antagonist in vitro. We have now tested the activity of this analog in vivo in rats with low or absent endogenous GH secretion. Surprisingly, treatment with G120RhGH failed to antagonize the effects of infusions or injections of hGH in hypophysectomized (Hx) rats and had little effect on hepatic GH-sensitive CYP2C transcripts in GH-deficient dwarf (dw) rats. Paradoxically, G120RhGH stimulated skeletal growth when infused into Hx rats; a pulsatile iv infusion was more effective than a continuous pattern. Coinfusion of G120RhGH with hGH produced an additive effect on growth. In addition, continuous, but not pulsatile, G120RhGH infusion elevated hepatic 2C12 messenger RNA (mRNA) expression and reduced 2C11 mRNA expression in Hx rats. The direct effects of G120RhGH on hepatic CYP2C transcripts were confirmed in cultured hepatocytes in vitro, which also revealed a significant action of PRL in elevating 2C12 mRNA expression. Binding studies revealed that G120RhGH bound preferentially to hepatic PRL receptors, as [125I]G120hGH was completely displaced by ovine PRL but was unaffected by bGH, a specific GH receptor ligand. The weak growth-promoting effects of G120RhGH were similar to those induced by recombinant hPRL in Hx rats. Our results show that G120RhGH is a poor in vivo GH antagonist in the rat, but shows a paradoxical agonist effect, probably mediated by PRL receptors in this species. |
| Databáze: | OpenAIRE |
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