Extracellular enzyme synthesis in a sporulation-deficient strain of Bacillus licheniformis
| Autor: | Fergus G. Priest, B Diderichsen, Alastair B. Fleming, P L Jørgensen, Martin Tangney |
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| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
DNA
Bacterial RNase P medicine.medical_treatment Mutant Molecular Sequence Data Bacillus Applied Microbiology and Biotechnology Plasmid medicine Bacillus licheniformis DNA Primers Spores Bacterial Protease Bacillaceae Ecology biology Base Sequence Serine Endopeptidases biology.organism_classification Bacillales Phenotype Biochemistry Genes Bacterial Amylases Xylanase Gene Deletion Food Science Biotechnology Research Article |
| Popis: | A deletion of the spoIIAC gene of Bacillus licheniformis was prepared in vitro by using the splicing-by-overlap-extension technique. This gene was introduced into B. licheniformis on a temperature-sensitive plasmid, and following integration and excision from the chromosome, a precisely located deletion on the chromosomal gene was prepared. The mutated bacterium was totally asporogenous and formed abortively disporic cells characterized by asymmetric septa at the poles of the cells. Qualitative plate tests revealed that the bacterium synthesized normal levels of DNase, polygalacturonate lyase, protease, RNase, and xylanase, but the hydrolysis zones due to beta-1,3-glucanase and carboxymethyl cellulase activity were smaller in the mutant than in the parent strain. The synthesis of alkaline protease was the same in batch cultures of the mutant and the parent during prolonged incubation for 72 h, but the alpha-amylase yields were reduced by about 30% by the mutation. |
| Databáze: | OpenAIRE |
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