A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull

Autor: Andrea Barbarossa, Anisa Bardhi, Teresa Gazzotti, Gaetano Mari, Giampiero Pagliuca
Přispěvatelé: Barbarossa, Andrea, Bardhi, Anisa, Gazzotti, Teresa, Mari, Gaetano, Pagliuca, Giampiero
Rok vydání: 2022
Předmět:
Zdroj: Drug Testing and Analysis. 14:1525-1531
ISSN: 1942-7611
1942-7603
DOI: 10.1002/dta.3270
Popis: Tulathromycin is a macrolide antibiotic generally used for the treatment of respiratory diseases in cattle and swine. This work proposes an improvement of a previously published LC-MS/MS method for tulathromycin determination in pig serum, here validated in three different bull matrices: plasma, seminal plasma, and urine. The approach is based on a quick protein precipitation with acetonitrile, filtration, and sample dilution before injection, allowing to rapidly process large batches of samples. Analytes separation was obtained using a BEH C18 (50 x 2.1 mm, 1.7 mu m) column, maintained at 40 degrees C with a chromatographic run of 5 min. The method was fully validated over concentration ranges suitable for field levels of tulathromycin found in each matrix (0.01-1 mu g/ml for plasma, 0.05-5 mu g/ml for seminal plasma, and 0.1-10 mu g/m1 for urine), showing good linearity during each day of testing (R-2 always >0.99). Accuracy and precision were within +/- 15% at all QC concentrations in all the three matrices. Furthermore, the use of tulathromycine-d7 as internal standard mitigated the potential impacts of matrix effect. The validated technique was successfully applied to samples collected during a pharmacokinetic study in bulls, allowing to monitor tulathromycin concentrations over time in the three matrices. To our knowledge, this is the first validated approach for LC-MS/MS quantification of tulathromycin in seminal plasma and urine.
Databáze: OpenAIRE
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