Masticatory hypofunction effects induced by BTXA injection of hippocampal neurons in developing rats
| Autor: | Fang Chun Wu, Hung Ming Chang, Chi Yang Tsai, Hsin Ping Lee |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Hippocampus Hippocampal formation CREB Random Allocation 03 medical and health sciences symbols.namesake 0302 clinical medicine Internal medicine medicine Animals Botulinum Toxins Type A Rats Wistar Cyclic AMP Response Element-Binding Protein General Dentistry Mastication Neurons biology business.industry 030206 dentistry Cell Biology General Medicine medicine.disease Immunohistochemistry Rats Masticatory force Endocrinology medicine.anatomical_structure Otorhinolaryngology biology.protein Nissl body symbols Neuron Alzheimer's disease business 030217 neurology & neurosurgery |
| Zdroj: | Archives of Oral Biology. 96:122-129 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2018.09.005 |
| Popis: | Background and objective In clinical practice, malocclusion is often encountered during the period of growth and development of individuals. In addition to nutritional imbalance, some studies have found that mastication affects learning and memory ability. Tooth loss and masticatory hypofunction have been suggested as risk factors of Alzheimer disease. However, relatively little research has been done in developing animals. The present study evaluated the relationship between masticatory hypofunction and neuropathological changes of the hippocampus in developing rats. Design Four-week-old Wistar rats were randomly divided into saline-injected and botulinum toxin type A (BTXA)-injected groups. After an experiment period of 4 weeks, the rats were sacrificed for evaluation of neuropathological changes in the hippocampus through Nissl staining and phosphorylated cyclic AMP (cAMP) response element binding protein (CREB) immunohistochemistry. Results Nissl staining revealed a significant reduction in the density of neurons in the BTXA-injected rats. The BTXA-injected rats exhibited a decreased level of CREB phosphorylation. The degree of p-CREB immunoreactivity differed significantly between the two groups. Conclusion The BTXA-injected rats exhibited a reduction in neuron density and phosphorylated CREB, indicating that mastication might influence the learning and memory ability during the growth period. Therefore, it is strongly suggested that malocclusion be corrected as soon as possible during growth and development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect