Gender differences in vascular expression of endothelin and ET A/ET B receptors, but not in calcium handling mechanisms, in deoxycorticosterone acetate-salt hypertension

Autor: Dorothy Nigro, Augusto C. Montezano, Rita C. Tostes, Zuleica Bruno Fortes, F. L. David, M. H. C. Carvalho, Nancy Amaral Rebouças
Jazyk: angličtina
Rok vydání: 2002
Předmět:
Male
Medicine (General)
Physiology
Biochemistry
chemistry.chemical_compound
General Pharmacology
Toxicology and Pharmaceutics
Biology (General)
Receptor
Desoxycorticosterone
Mesenteric arteries
lcsh:QH301-705.5
Sex Characteristics
lcsh:R5-920
Endothelin-1
Receptors
Endothelin
Reverse Transcriptase Polymerase Chain Reaction
DOCA-salt hypertension
General Neuroscience
General Medicine
Receptor
Endothelin A
Bay K8644
Receptor
Endothelin B
medicine.anatomical_structure
Hypertension
Female
medicine.symptom
Endothelin receptor
lcsh:Medicine (General)
medicine.medical_specialty
QH301-705.5
Immunology
Biophysics
Biology
Endothelin receptors
R5-920
Internal medicine
Caffeine
medicine
Animals
RNA
Messenger
Rats
Wistar
Calcium metabolism
Gender
Cell Biology
3-Pyridinecarboxylic acid
1
4-dihydro-2
6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-
Methyl ester
Endothelin 1
Rats
Calcium Channel Agonists
Endocrinology
chemistry
lcsh:Biology (General)
Vasoconstriction
Calcium
Serotonin
Zdroj: Brazilian Journal of Medical and Biological Research, Vol 35, Iss 9, Pp 1061-1068 (2002)
Brazilian Journal of Medical and Biological Research v.35 n.9 2002
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Volume: 35, Issue: 9, Pages: 1061-1068, Published: SEP 2002
Popis: We determined if the increased vascular responsiveness to endothelin-1 (ET-1) observed in male, but not in female, DOCA-salt rats is associated with differential vascular mRNA expression of ET-1 and/or ET A/ET B receptors or with functional differences in Ca2+ handling mechanisms by vascular myocytes. Uninephrectomized male and female Wistar rats received DOCA and drinking water containing NaCl/KCl. Control rats received vehicle and tap water. Blood pressure and contractile responses of endothelium-denuded aortic rings to agents which induce Ca2+ influx and/or its release from internal stores were measured using standard procedures. Expression of mRNA for ET-1 and ET A/ET B receptors was evaluated by RT-PCR after isolation of total cell RNA from both aorta and mesenteric arteries. Systolic blood pressure was higher in male than in female DOCA rats. Contractions induced by Bay K8644 (which activates Ca2+ influx through voltage-operated L-type channels), and by caffeine, serotonin or ET-1 in Ca2+-free buffer (which reflect Ca2+ release from internal stores) were significantly increased in aortas from male and female DOCA-salt compared to control aortas. DOCA-salt treatment of male, but not female, rats statistically increased vascular mRNA expression of ET-1 and ET B receptors, but decreased the expression of ET A receptors. Molecular up-regulation of vascular ET B receptors, rather than differential changes in smooth muscle Ca2+ handling mechanisms, seems to account for the increased vascular reactivity to ET-1/ET B receptor agonists and higher blood pressure levels observed in male DOCA-salt rats.
Databáze: OpenAIRE
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