In vitro and Short-term in vivo Characteristics of a Kel-F Thin Film Modified Glucose Sensor
| Autor: | Sunmin Park, Sun Kil Kang, Hee Chan Kim, Se Jin Park, Ran-A Jeong, Taek Dong Chung |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Blood Glucose
Male Time Factors Analytical chemistry chemistry.chemical_element Biosensing Techniques Oxygen Analytical Chemistry Rats Sprague-Dawley Glucose Oxidase chemistry.chemical_compound Nafion Animals Glucose oxidase Thin film Composite material Electrodes Polytetrafluoroethylene chemistry.chemical_classification biology Reproducibility of Results Polymer Rats Fluorocarbon Polymers chemistry Electrode Microscopy Electron Scanning biology.protein Layer (electronics) |
| Zdroj: | Analytical Sciences. 19:1481-1486 |
| ISSN: | 1348-2246 0910-6340 |
| DOI: | 10.2116/analsci.19.1481 |
| Popis: | A new outer layer composition, consisting of polytetrafluoroethylene (PTFE), Kel-F oil, and Nafion, is suggested to minimize the detrimental effect of dissolved oxygen and to extend the linear response range of a glucose oxidase(GOx)-based sensor using nonconducting polymer. The morphology of Kel-F/PTFE/Kel-F/Nafion polymeric laminate was followed during fabrication by SEM. When Kel-F film was formed on the PTFE outer layer, the linear response was extended to 21 mM, at a sensitivity of 2.8 +/- 0.8 nA/mM mm2. We demonstrate that a sensor without Kel-F/PTFE/Kel-F/Nafion outer layer is relatively oxygen dependent, whereas by comparison a sensor with Kel-F/PTFE/Kel-F/Nafion outer layer is oxygen independent. The current of such a glucose sensor implanted in the subcutaneous tissue stabilized within 60 min, and the lag between blood glucose changes and sensor output was within 1 min. The in vivo characteristics of the glucose sensor described show great promise for one-point in vivo calibration. |
| Databáze: | OpenAIRE |
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