Reproducibility of sublingual microcirculation parameters obtained from sidestream darkfield imaging
| Autor: | Sara-Joan Pinto-Sietsma, Aeilko H. Zwinderman, Luca Valerio, Ron J.G. Peters |
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| Přispěvatelé: | Graduate School, Cardiology, Public and occupational health, ACS - Atherosclerosis & ischemic syndromes, APH - Methodology, Epidemiology and Data Science, APH - Health Behaviors & Chronic Diseases, ACS - Heart failure & arrhythmias |
| Rok vydání: | 2018 |
| Předmět: |
Male
Research Validity Erythrocytes Intraclass correlation Physiology Video Recording Social Sciences 030204 cardiovascular system & hematology Cardiovascular Physiology Vascular Medicine Habits 0302 clinical medicine Animal Cells Red Blood Cells Medicine and Health Sciences Smoking Habits Medicine Psychology Coronary Heart Disease 030212 general & internal medicine Longitudinal Studies Reliability (statistics) Erythrocytes/physiology Multidisciplinary Middle Aged Research Assessment Reproducibility Cardiovascular Diseases Blood Circulation Female Cellular Types Research Article Video Recording/methods Adult Imaging Techniques Science Cardiology Cardiovascular Diseases/pathology Research and Analysis Methods Glycocalyx Microcirculation 03 medical and health sciences Statistical significance Humans Mouth Floor Behavior Mouth Floor/blood supply Blood Cells business.industry Surrogate endpoint Microcirculation/physiology Reproducibility of Results Biology and Life Sciences Cell Biology Standard error Sample size determination business Nuclear medicine |
| Zdroj: | PLoS ONE PLoS ONE, 14(3). Public Library of Science PLoS ONE, Vol 14, Iss 3, p e0213175 (2019) |
| ISSN: | 1932-6203 |
| Popis: | BackgroundChanges in the microcirculation may be used as a surrogate outcome in studies on cardiovascular disease. We assessed the reliability characteristics of the sublingual microcirculation parameters Vascular Density (VD), Red Blood Cell Filling (RBCF), and Perfused Boundary Region (PBR) as obtained by sidestream darkfield imaging.MethodsFor each of the three parameters, the variance components of measurement, the Intraclass Correlation Coefficient (ICC), the Standard Error of Measurement, and the limits of agreement were estimated for the intra-rater setting (N = 50) and the inter-rater setting (N = 48). Subsequently, as a proof of concept, the reliability measures were used for a power analysis to design studies to evaluate the effect of acute stimuli-i.e. having a meal (N = 50) and cigarette smoking (N = 21) on the three parameters.ResultsReproducibility was poor for all three parameters. The intra-rater ICC for 2 measurements was 0.28 (95% CI: 0.04, 0.53) for the VD, 0.51 (95% CI: 0.27, 0.69) for the RBCF, and 0.33 (95% CI: 0.08-0.56) for the PBR. The standard errors of measurement and the limits of agreement for all three parameters were larger than most statistically significant intra-individual or inter-individual differences reported in previous studies. The proofs of concept showed that sample sizes in excess of 600 subjects are necessary to reach statistical significance for the observed effects of having a meal or smoking on VD and PBR.ConclusionsThe reliability of the three sublingual microcirculation parameters in their current form appears to be low and a large sample size is advisable for their use in conditions similar to those we describe. |
| Databáze: | OpenAIRE |
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