Hepatic and Plasma Endothelin-1 in Dogs with Chronic Hepatitis
| Autor: | Y. Sakamoto, Manabu Sakai, Toshihiro Watari |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Cirrhosis 040301 veterinary sciences Standard Article Chronic liver disease Canine 0403 veterinary science Pathogenesis 03 medical and health sciences Dogs Internal medicine medicine Animals Dog Diseases Prospective Studies Portal hypertension Hepatitis Chronic Vasoactive peptide Endothelin-1 Hepatology General Veterinary business.industry 04 agricultural and veterinary sciences medicine.disease Endothelin 1 Standard Articles Reverse transcription polymerase chain reaction 030104 developmental biology Endocrinology Case-Control Studies Female SMALL ANIMAL Splenic Pulp Endothelin receptor business |
| Zdroj: | Journal of Veterinary Internal Medicine |
| ISSN: | 0891-6640 |
| Popis: | Background Endothelin (ET)-1 is a 21-amino-acid peptide with potent vasoactive properties, which increases intrahepatic resistance in patients with chronic hepatitis (CH) or cirrhosis. ET-1 concentrations have not been investigated in dogs with CH. Hypothesis/Objectives This study compared hepatic and plasma ET-1 levels in healthy dogs and in dogs with CH, and examined the relationship between the plasma ET-1 level and portal vein pressure in dogs with CH. Animals Fourteen healthy dogs and twenty dogs with CH were used in this study. Methods Prospective case-control study. Hepatic ET-1 mRNA expression was determined by real-time reverse transcription polymerase chain reaction, and hepatic and plasma ET-1 levels were assessed using ELISA. Splenic pulp pressure (SPP), as an indicator of portal vein pressure, was measured laparoscopically. Results Hepatic ET-1 mRNA levels were 3.7 times higher in dogs with CH than in healthy dogs (P = .008). The median hepatic and plasma ET-1 protein levels were significantly higher in dogs with CH than in healthy dogs (13.20 pg/mg wet liver vs. 3.42 pg/mg wet liver, P = .004, and 0.99 pg/mL vs. 0.71 pg/mL, P = .013, respectively). Moreover, there was a weak but significant correlation between plasma ET-1 level and SPP in dogs with CH (P = .036; rs = 0.53). Conclusions and clinical importance The results indicate that ET-1 might play an important role in the pathogenesis of portal hypertension caused by CH. |
| Databáze: | OpenAIRE |
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