In-situhybridization-based quantification of hTR: a possible biomarker in malignant melanoma
| Autor: | Josephine Vagner, Magnus Stougaard, Torben Steiniche |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Telomerase
Skin Neoplasms Histology Tissue microarray Melanoma General Medicine In situ hybridization Biology medicine.disease Immunohistochemistry Molecular biology Pathology and Forensic Medicine Breslow Thickness Biomarkers Tumor medicine Humans RNA Biomarker (medicine) Telomerase reverse transcriptase neoplasms Nevus In Situ Hybridization Predictive biomarker |
| Zdroj: | Vagner, J, Steiniche, T & Stougaard, M 2015, ' In-situ hybridization based quantification of hTR: a possible biomarker in malignant melanoma ', Histopathology, vol. 66, no. 5 . https://doi.org/10.1111/his.12501 |
| ISSN: | 0309-0167 |
| Popis: | Aims Telomerase is reactivated in most cancers and there is accumulating evidence that this is a driver event in malignant melanoma (MM). Thus, our aim was to evaluate if in-situ hybridization (ISH)-based quantification of telomerase RNA (hTR) could be used to distinguish MM from naevi, and if there was a correlation with the Breslow thickness. Results and methods We created a tissue microarray (TMA) from formalin-fixed and paraffin-embedded tissue samples from 17 MM and 23 naevi, performed ISH targeting hTR, and quantified the signals. We found a more than eightfold greater number of hTR signals per nucleus in the MM samples compared to the naevi, and a positive correlation (P = 0.0381) between the number of hTR signals per nucleus and the Breslow thickness. Conclusion Quantification of hTR ISH signals clearly distinguish MM from naevi (P |
| Databáze: | OpenAIRE |
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