Enhanced cross protection by hetero prime-boost vaccination with recombinant influenza viruses containing chimeric hemagglutinin-M2e epitopes
| Autor: | Bo Ryoung Park, Jeeva Subbiah, Ki-Hye Kim, Young-Man Kwon, Judy Oh, Min-Chul Kim, Chong-Hyun Shin, Baik Lin Seong, Sang-Moo Kang |
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| Rok vydání: | 2022 |
| Předmět: |
Models
Molecular Aging Cross Protection Recombinant Fusion Proteins Immunization Secondary Hemagglutinin Glycoproteins Influenza Virus Antibodies Viral Article Viral Matrix Proteins Mice Immunogenicity Vaccine Influenza A Virus H1N1 Subtype Orthomyxoviridae Infections Virology Influenza Human Animals Humans Antigens Viral Vaccines Synthetic Influenza A Virus H3N2 Subtype Vaccination Age Factors Mice Inbred C57BL Influenza Vaccines Immunoglobulin G Female |
| Zdroj: | Virology |
| ISSN: | 0042-6822 |
| Popis: | Annual repeat influenza vaccination raises concerns about protective efficacy against mismatched viruses. We investigated the impact of heterologous prime-boost vaccination on inducing cross protection by designing recombinant influenza viruses with chimeric hemagglutinin (HA) carrying M2 extracellular domains (M2e-HA). Heterologous prime-boost vaccination of C57BL/6 mice with M2e-HA chimeric virus more effectively induced M2e and HA stalk specific IgG antibodies correlating with cross protection than homologous prime-boost vaccination. Induction of M2e and HA stalk specific IgG antibodies was compromised in 1-year old mice, indicating significant aging effects on priming subdominant M2e and HA stalk IgG antibody responses. This study demonstrates that a heterologous prime-boost strategy with recombinant influenza virus expressing extra M2e epitopes provides more effective cross protection than homologous vaccination. |
| Databáze: | OpenAIRE |
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