Correlation of free radical level and apoptosis after intracerebral hemorrhage in rats
| Autor: | Tao Wu, You-Li Zhu, Su-Ju Ding, Ning Han |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Physiology Apoptosis Rats sprague dawley Rats Sprague-Dawley chemistry.chemical_compound Random Allocation Malondialdehyde Edaravone medicine In Situ Nick-End Labeling Animals cardiovascular diseases Neuronal apoptosis Cerebral Hemorrhage Intracerebral hemorrhage Analysis of Variance TUNEL assay Dose-Response Relationship Drug business.industry Hydroxyl Radical General Neuroscience General Medicine Human physiology Free Radical Scavengers medicine.disease nervous system diseases Rats Disease Models Animal chemistry Anesthesia Linear Models Original Article business Antipyrine |
| Zdroj: | Neuroscience bulletin. 24(6) |
| ISSN: | 1673-7067 |
| Popis: | To investigate the correlation of perihematomal free radical level and neuronal apoptosis following the intracerebral hemorrhage (ICH).Animals were randomly divided into 4 groups: sham operation group, model group, 1 mg/kg edaravone group, and 3 mg/kg edaravone group. Each group was then divided into seven subgroups, in which the rats were correspondingly killed at 6 h, 12 h, 24 h, 48 h, 72 h, 7 d or 14 d (n = 1 in each subgroup of the sham group, and n = 6 in each subgroup of the other 3 groups). By Horseley-Clarke technique, autoblood (80 microL) were administered into the left caudate putamen of SD rats in a double administration-withdrawal way. Rats in the sham group were needled in but not administered with autoblood. The ICH model was then evaluated by Bederson's scale. Around the hematoma, the levels of malonaldehyde (MDA) and hydroxyl radical were tested by spectrophotometer, and the process of apoptosis was tested by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) method.(1) ICH significantly increased the levels of MDA and hydroxyl radicals. Significant differences in MDA and hydroxyl radical contents were observed among the four groups. (2) In the sham group, a small number of TUNEL-positive cells were found. In the other three groups, the TUNEL-positive cells were observed at 6 h, increased significantly at 24 h, and reached peak level at 3 d, then fell profoundly at 7 d, but remained detectable at 14 d. (3) The positive correlation existed between apoptosis and free radical level (r = 0.2003), and existed between apoptosis and MDA content (r = 0.6563) in the brain.Post-hemorrhagic apoptosis was related to the production of free radicals, indicating that the elevated free radicals following the ICH could induce neuron and glial cell apoptosis. |
| Databáze: | OpenAIRE |
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