Functional overlap of GATA-1 and GATA-2 in primitive hematopoietic development
| Autor: | Aaron N. Chang, Stuart H. Orkin, Yuko Fujiwara, Aimée M. Williams |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Immunology Mice Transgenic Biology Biochemistry Mice Testis medicine Animals Humans GATA1 Transcription Factor Yolk sac Transcription factor Crosses Genetic Yolk Sac Mice Knockout Genetics Embryogenesis GATA2 Embryo Cell Biology Hematology Embryonic stem cell Hematopoiesis Cell biology DNA-Binding Proteins GATA2 Transcription Factor Haematopoiesis medicine.anatomical_structure embryonic structures Erythroid-Specific DNA-Binding Factors Female Stem cell Transcription Factors |
| Zdroj: | Blood. 103:583-585 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2003-08-2870 |
| Popis: | Transcription factors GATA-1 and GATA-2 are required for normal hematopoiesis. The loss of either leads to embryonic lethality in knockout mice because of the failure of erythroid maturation and the expansion of progenitors, respectively. As the expression of GATA-1 and GATA-2 overlaps within hematopoietic progenitors, the extent to which these factors functionally compensate for each other during embryogenesis is unknown. As shown here, we have analyzed double-knockout embryos at the yolk sac stage of development and have shown that the combined absence of these GATA factors virtually ablates primitive erythroid cell formation. Thus, the function of GATA-1 and GATA-2 overlaps at the yolk sac stage. Moreover, a GATA factor, either GATA-1 or GATA-2, is required to initiate blood formation in the embryo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|