Development, validation and application of a GC-MS method for the simultaneous detection and quantification of neutral lipid species in Trypanosoma cruzi
| Autor: | Hugo Cerecetto, Mercedes González, Elsa Moreno-Viguri, Elena Lizarraga, Silvia Pérez-Silanes, Mery Santivañez-Veliz, Javier Varela |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Squalene Trypanosoma cruzi 030231 tropical medicine Clinical Biochemistry Sterol Biosynthesis Pathway Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Lanosterol 0302 clinical medicine Limit of Detection Ergosterol parasitic diseases Squalene-2 3-epoxide Animals Chromatography biology Reproducibility of Results Cell Biology General Medicine biology.organism_classification Neutral lipid Sterols 030104 developmental biology chemistry Linear Models Gas chromatography–mass spectrometry GC-MS |
| Zdroj: | Dadun. Depósito Académico Digital de la Universidad de Navarra instname |
| Popis: | The development and validation of an analytical method for the simultaneous analysis of five neutral lipids in Trypanosoma cruzi epimastigotes by GC-MS is presented in this study. The validated method meets all validation parameters for all components and the chromatographic conditions have been optimized during its development. This analytical method has demonstrated good selectivity, accuracy, within-day precision, recovery and linearity in each of the established ranges. In addition, detection and quantification limits for squalene, cholesterol, ergosterol and lanosterol have been improved and it is worth highlighting the fact that this is the first time that squalene-2,3-epoxide validation data have been reported. The new validated method has been applied to epimastigotes treated with compounds with in vitro anti- T.cruzi activity. This new methodology is straightforward and constitutes a tool for screening possible sterol biosynthesis pathway inhibitors in Trypanosoma cruzi, one of the most studied targets in Chagas disease treatment. Therefore, it is an interesting and useful contribution to medicinal chemistry research. |
| Databáze: | OpenAIRE |
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