Alteration of the gut microbiota in Chinese population with chronic kidney disease
| Autor: | Jing Nie, Hanchang He, Dan Lv, Pu Wang, Qian-Yun Lin, Hongwei Zhou, Fan Fan Hou, Shuanghong Jiang, Jianping Jiang, Youlian Zhou, Shan Xie, Ting Zhang, Ye Chen |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine China Science 030232 urology & nephrology Faecalibacterium prausnitzii Gut flora Kidney Function Tests urologic and male genital diseases Article End stage renal disease Microbiology 03 medical and health sciences 0302 clinical medicine Prevotella Humans Renal Insufficiency Chronic Aged Multidisciplinary Bacteria biology Computational Biology Middle Aged biology.organism_classification Gastrointestinal Microbiome Butyrates 030104 developmental biology Cystatin C Case-Control Studies Population Surveillance biology.protein Kidney Failure Chronic Metagenome Medicine Female Enterotype Metagenomics Bacteroides Roseburia Biomarkers |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-02989-2 |
| Popis: | We evaluated differences in the compositions of faecal microbiota between 52 end stage renal disease (ESRD) patients and 60 healthy controls in southern China using quantitative real-time polymerase chain reaction (qPCR) and high-throughput sequencing (16S ribosomal RNA V4-6 region) methods. The absolute quantification of total bacteria was significantly reduced in ESRD patients (p Prevotella was enriched in the healthy group whereas Bacteroides were prevalent in the ESRD group (LDA score > 4.5). 11 bacterial taxa were significantly overrepresented in samples from ESRD and 22 bacterial taxa were overrepresented in samples from healthy controls. The butyrate producing bacteria, Roseburia, Faecalibacterium, Clostridium, Coprococcus and Prevotella were reduced in the ESRD group (LDA values > 2.0). Canonical correspondence analysis (CCA) indicated that Cystatin C (CysC), creatinine and eGFR appeared to be the most important environmental parameters to influence the overall microbial communities. In qPCR analysis, The butyrate producing species Roseburia spp., Faecalibacterium prausnitzii, Prevotella and Universal bacteria, were negatively related to CRP and CysC. Total bacteria in faeces were reduced in patients with ESRD compared to that in healthy individuals. The enterotypes change from Prevotella to Bacteroides in ESRD patients. The gut microbiota was associated with the inflammatory state and renal function of chronic kidney disease. |
| Databáze: | OpenAIRE |
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