Acute Phase Protein Levels and Thymus, Spleen and Plasma Protein Synthesis Rates Differ in Adult and Old Rats
| Autor: | J. Prugnaud, Claire Sornet, Christiane Obled, Corinne Pouyet, Dominique Dardevet, Isabelle Papet, Fabienne Béchereau |
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| Přispěvatelé: | Unité de nutrition et métabolisme protéique, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Rok vydání: | 2003 |
| Předmět: |
Male
Aging medicine.medical_specialty 030309 nutrition & dietetics Medicine (miscellaneous) Spleen Inflammation Thymus Gland Biology 03 medical and health sciences Internal medicine Blood plasma medicine Animals Rats Wistar ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Nutrition and Dietetics Albumin Acute-phase protein Blood Proteins Organ Size Immunosenescence Blood proteins Rats [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition Lymphatic system medicine.anatomical_structure Endocrinology RAT medicine.symptom [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Acute-Phase Proteins |
| Zdroj: | Scopus-Elsevier HAL Journal of Nutrition Journal of Nutrition, American Society for Nutrition, 2003, 133 (1), pp.215-219 |
| ISSN: | 0022-3166 |
| DOI: | 10.1093/jn/133.1.215 |
| Popis: | Aging induces a dysregulation of immune and inflammation functions that may affect protein synthesis rates in lymphoid tissue and plasma proteins. We quantified in vivo synthesis rates of thymus, spleen and plasma proteins, including albumin and acute phase proteins, in adult (8 mo old) and old (22 mo old) rats using the flooding dose method [L-(1-(13)C) phenylalanine]. Immunosenescence was reflected by thymus atrophy and spleen hypertrophy in old rats but not in adult rats. A low albumin plasma level associated with high concentrations of fibrinogen, alpha(2)-macroglobulin, alpha(1)-acid glycoprotein and proteins other than albumin revealed a low grade inflammation in old rats. Protein fractional synthesis rates (FSR) and protein synthesis efficiencies of thymus were 29 and 26% lower in old than in adult rats, respectively; these variables did not differ in spleen. Protein absolute synthesis rates (ASR) of the thymus and spleen were 76% lower and 67% greater in old than adult rats, respectively. The FSR and ASR of albumin and other plasma proteins were greater in old than in adult rats. Protein synthesis measurement is a valuable nonimmunological tool to assess, in vivo, immune and inflammatory variables. Alterations in secondary lymphoid organs and plasma protein synthesis may contribute to the significant repartitioning of amino acids in old compared with adult rats and may be involved in sarcopenia. |
| Databáze: | OpenAIRE |
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