Additional file 1 of Modeling early germline immunization after horizontal transfer of transposable elements reveals internal piRNA cluster heterogeneity

Autor: Asif-Laidin, Amna, Casier, Karine, Ziriat, Zoheir, Boivin, Antoine, Viodé, Elise, Delmarre, Valérie, Ronsseray, Stéphane, Carré, Clément, Teysset, Laure
Rok vydání: 2023
DOI: 10.6084/m9.figshare.23167799.v1
Popis: Additional file 1: Fig. S1. Mapping of complementary maternally inherited piRNAs on a piRNA cluster locus. Fig. S2. Parental strains and experimental schemes. Fig. S3. Concomitant conversion of all the regions of the P transgene in the MI H and PI B sublines. Fig. S4. Conversion of cluster1A in the Δ-1Aw1118 background. Fig. S5. Conversion of the P transgene inserted in the autosomal cluster100F. Fig. S6. P-derived sequences of P are able to repress ovarian expression of pRFP. Fig. S7. Ovarian lacZ silencing induced by P is impaired by germline knockdown of genes involved in piRNA biology. Fig. S8. Crosses used for the conversion of the P transgenes by P or P. Fig. S9. Study of composition of converted sequences. Fig. S10. No cis-conversion of flanking regions of the P transgene clusters or of cluster 1A. Fig. S11. No trans-conversion of endogenous homologous sequences. Fig. S12. RT-qPCR experiments of P and T3 in P-1152. Fig. S13. siRNAs and piRNAs abundance during conversion of cluster 1A. Fig. S14. Comparison of methods for small RNA libraries normalizations.
Databáze: OpenAIRE