The alternative splicing of BdTai and its involvement in the development of Bactrocera dorsalis (Hendel)
| Autor: | Hong Liu, Yong Yue, Wei Dou, Hui-Min Li, Zhong-Hao Song, Jin-Jun Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Gene isoform DNA Complementary Physiology media_common.quotation_subject Methoprene 01 natural sciences Bactrocera dorsalis 03 medical and health sciences chemistry.chemical_compound Botany Animals Protein Isoforms Amino Acid Sequence RNA Messenger Metamorphosis Cloning Molecular Phylogeny media_common Larva Gene knockdown biology fungi Tephritidae Pupa Gene Expression Regulation Developmental biology.organism_classification Cell biology Juvenile Hormones 010602 entomology Alternative Splicing 030104 developmental biology chemistry Insect Science Juvenile hormone Insect Proteins Sequence Alignment Ecdysone Signal Transduction |
| Zdroj: | Journal of insect physiology. 101 |
| ISSN: | 1879-1611 |
| Popis: | Interest in insect metamorphosis has primarily focused on juvenile hormone (JH) and ecdysone. Compared to ecdysone signaling, the molecular action of JH is less well established because Methoprene-tolerant (Met) as the JH receptor has been identified until recently. In vitro studies have indicated that Met forms an active JH-dependent complex with one partner protein, Taiman (Tai). However, the related studies on Tai’s role in insect metamorphosis are very limited. In this study, five Tai isoforms differing in C-terminal region are identified from the oriental fruit fly Bactrocera dorsalis, an important worldwide pest infesting fruits and vegetables. The spatiotemporal expression pattern analysis indicates that BdTai-A and BdTai-B are highly expressed in early larvae while BdTai-D is more abundant in middle-late larvae. Meanwhile, in vivo methoprene stress leads to dramatic expression pattern fluctuation of BdTai isoforms. The subsequent reverse genetic study reveal that all Tai isoforms (denoted as “Tai-core”) depletion in larvae stage of B. dorsalis produce precocious larvae-pupae development, i.e. shortened pupation process and miniature pupae. Further knockdown of individual Tai isoform show that silence of BdTai-E causes the same phenotype as of BdTai-core RNAi. The current data suggest that BdTai-E is involved in transducing the JH signal that represses metamorphosis. Besides, isoforms should be considered when studying Tai functions. |
| Databáze: | OpenAIRE |
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