Ammonia uptake by transmembrane pH gradient poly(isoprene)-block-poly(ethylene glycol) polymersomes
| Autor: | Charlotte Weitzel, Jean-Christophe Leroux, Yinyin Bao, Schmidt Aaron Christoph, Karl-Heinz Altmann, Barbara Stoessel, Erik R. Hebels |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Polymers
Metabolite 02 engineering and technology 010402 general chemistry 01 natural sciences Polyethylene Glycols Polymerization chemistry.chemical_compound Ammonia Hemiterpenes PEG ratio Butadienes medicine Humans Particle Size Urea Cycle Disorders Inborn Drug Carriers Chemistry Liver Diseases technology industry and agriculture Proton-Motive Force Water Hyperammonemia General Chemistry Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Condensed Matter Physics medicine.disease 0104 chemical sciences Hepatic Encephalopathy Urea cycle Inactivation Metabolic Polymersome Biophysics Methacrylates 0210 nano-technology Ethylene glycol Fluorescein-5-isothiocyanate |
| Zdroj: | Soft Matter, 16 (11) |
| ISSN: | 1744-683X 1744-6848 |
| Popis: | Transmembrane pH gradient poly(isoprene)-block-poly(ethylene glycol) (PI-b-PEG) polymersomes were investigated for their potential use in the detoxification of ammonia, a metabolite that is excessively present in patients suffering from urea cycle disorders and advanced liver diseases, and which causes neurotoxic effects (e.g., hepatic encephalopathy). Polymers varying in PI and PEG block length were synthesized via nitroxide-mediated polymerization and screened for their ability to self-assemble into polymersomes in aqueous media. Ammonia sequestration by the polymersomes was investigated in vitro. While most vesicular systems were able to capture ammonia in simulated intestinal fluids, uptake was lost in partially dehydrated medium mimicking conditions in the colon. Polymeric crosslinking of residual olefinic bonds in the PI block increased polymersome stability, partially preserving the ammonia capture capacity in the simulated colon environment. These more stable vesicular systems hold promise for the chronic oral treatment of hyperammonemia. Soft Matter, 16 (11) ISSN:1744-683X ISSN:1744-6848 |
| Databáze: | OpenAIRE |
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