Relocating Single Molecules in Super-Resolved Fluorescence Lifetime Images near a Plasmonic Nanostructure
| Autor: | Angelo Gulinatti, Ignacio Izeddin, Valentina Krachmalnicoff, Yannick De Wilde, Guillaume Blanquer, Giulia Acconcia, Bart van Dam |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Point spread function
Fluorescence-lifetime imaging microscopy Materials science point spread function (PSF) Field of view 02 engineering and technology local density of optical states (LDOS) 01 natural sciences mislocalization 010309 optics 0103 physical sciences Microscopy single-molecule localization microscopy (SMLM) Electrical and Electronic Engineering Nanoscopic scale Common emitter decay rate business.industry Orientation (computer vision) 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Dipole Optoelectronics 0210 nano-technology business Biotechnology single-molecule fluorescence lifetime imaging microscopy (smFLIM) |
| Popis: | Single-molecule localization microscopy is a powerful technique with vast potential to study light-matter interactions at the nanoscale. Nanostructured environments can modify the fluorescence emission of single molecules and the induced decay-rate modification can be retrieved to map the local density of optical states (LDOS). However, the modification of the emitter’s point spread function (PSF) can lead to its mislocalization, setting a major limitation to the reliability of this approach. In this paper, we address this by simultaneously mapping the position and decay rate of single-molecules and by sorting events by their decay rate and PSF size. With the help of numerical simulations, we are able to infer the dipole orientation and to retrieve the real position of mislocalized emitters. We have applied our ap-proach of single-molecule fluorescence lifetime imaging microscopy (smFLIM) to study the LDOS modification of a silver nanowire over a field of view of ~10 µm2 with a single-molecule localizatio... |
| Databáze: | OpenAIRE |
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