Application of mid-infrared microscopic imaging for the diagnosis and classification of human lymphomas
| Autor: | Seraphin Hubert Unterberger, Christian W. Huck, Roland Stalder, Ella Willenbacher, Wolfgang Willenbacher, Andrea Brunner, Bettina Zelger, J. D. Pallua |
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| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Proliferative index Follicular lymphoma General Physics and Astronomy Aggressive lymphoma 01 natural sciences General Biochemistry Genetics and Molecular Biology 010309 optics hemic and lymphatic diseases PD-L1 0103 physical sciences Medicine Humans General Materials Science biology business.industry 010401 analytical chemistry General Engineering General Chemistry medicine.disease Subtyping 0104 chemical sciences Lymphoma biology.protein Immunohistochemistry Lymph Nodes Lymphoma Large B-Cell Diffuse business Diffuse large B-cell lymphoma |
| Zdroj: | Journal of biophotonicsREFERENCES. 14(9) |
| ISSN: | 1864-0648 |
| Popis: | Mid-infrared (MIR) microscopic imaging of indolent and aggressive lymphomas was performed including formalin-fixed and paraffin-embedded samples of six follicular lymphomas and 12 diffuse large B-cell-lymphomas as well as reactive lymph nodes to investigate benefits and challenges for lymphoma diagnosis. MIR images were compared to defined pathological characteristics such as indolent versus aggressive versus reactive, germinal centre versus activated cell-of-origin (COO) subtypes, or a low versus a high proliferative index and level of PD-L1 expression. We demonstrated that MIR microscopic imaging can differentiate between reactive lymph nodes, indolent and aggressive lymphoma samples. Also, it has potential to be used in the subtyping of lymphomas, as shown with the differentiation between COO subtypes, the level of proliferation and PD-L1 expression. MIR microscopic imaging is a promising tool for diagnosis and subtyping of lymphoma and further evaluation is needed to fully explore the advantages and disadvantages of this method for pathological diagnosis. |
| Databáze: | OpenAIRE |
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