Regulation of nonylphenol-induced reproductive toxicity in mouse spermatogonia cells by miR-361-3p
| Autor: | Hui Zhang, Wenbing Yuan, Bo Zhao, Qiao Du, Shaohua Bian, Cheng Luo, Zhijiang Zhao, Hang Bi, Ce Chen, Huimin Sun, Qiyuan Kang, Jiang Zhu, Liyan Tang, Yingyi Li |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Untranslated region Male Down-Regulation Apoptosis Cell Line 03 medical and health sciences Mice Phenols In vivo Puma Genetics Animals p53 upregulated modulator of apoptosis Transcription factor 3' Untranslated Regions Messenger RNA biology Tumor Suppressor Proteins Tumor Protein p73 Cell Biology biology.organism_classification Molecular biology Spermatogonia MicroRNAs 030104 developmental biology Cell culture biology.protein Apoptosis Regulatory Proteins Developmental Biology |
| Zdroj: | Molecular reproduction and development. 84(12) |
| ISSN: | 1098-2795 |
| Popis: | Nonylphenol (NP) is an environmental chemical that affects apoptosis and male infertility. In our study, we found that a high concentration of NP could down-regulate the expression of microRNA-361-3p (miR-361-3p) in the murine GC-1 spermatogonia cell line and in vivo in murine spermatogonia. Additionally, one direct target of this miR, the 3' untranslated region of Killin (Klln) mRNA, was identified. Klln encodes a transcription factor that directly regulates the expression of Tp73 (transcriptionally active p73), whose encoded protein can up-regulate the expression of Puma (p53 upregulated modulator of apoptosis). Thus, our investigation revealed that the expression of Klln, Tp73, and Puma increased upon NP-dependent down-regulation of miR-361-3p, which eventually leads to apoptosis of spermatogonia. |
| Databáze: | OpenAIRE |
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