Barcoded pyrosequencing analysis of the microbial community in a simulator of the human gastrointestinal tract showed a colon region-specific microbiota modulation for two plant-derived polysaccharide blends
| Autor: | Robert Sinnott, Frederiek-Maarten Kerckhof, Sam Possemiers, Gabriela Luta, Nico Boon, Massimo Marzorati, Tom Van de Wiele, An Verhelst, Lois Maignien |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Colon
Population Microbial metabolism Biology Gut flora Microbiology Polymerase Chain Reaction Polysaccharides medicine DNA Barcoding Taxonomic Humans Aloe education Molecular Biology Simulation education.field_of_study Bacteria Denaturing Gradient Gel Electrophoresis Dendrogram Human gastrointestinal tract General Medicine Sequence Analysis DNA Models Theoretical biology.organism_classification Biota medicine.anatomical_structure Microbial population biology Pyrosequencing Temperature gradient gel electrophoresis |
| Zdroj: | Antonie van Leeuwenhoek. 103(2) |
| ISSN: | 1572-9699 |
| Popis: | The combination of a Simulator of the Human Intestinal Microbial Ecosystem with ad hoc molecular techniques (i.e. pyrosequencing, denaturing gradient gel electrophoresis and quantitative PCR) allowed an evaluation of the extent to which two plant polysaccharide supplements could modify a complex gut microbial community. The presence of Aloe vera gel powder and algae extract in product B as compared to the standard blend (product A) improved its fermentation along the entire simulated colon. The potential extended effect of product B in the simulated distal colon, as compared to product A, was confirmed by: (i) the separate clustering of the samples before and after the treatment in the phylogenetic-based dendrogram and OTU-based PCoA plot only for product B; (ii) a higher richness estimator (+33 vs. -36 % of product A); and (iii) a higher dynamic parameter (21 vs. 13 %). These data show that the combination of well designed in vitro simulators with barcoded pyrosequencing is a powerful tool for characterizing changes occurring in the gut microbiota following a treatment. However, for the quantification of low-abundance species-of interest because of their relationship to potential positive health effects (i.e. bifidobacteria or lactobacilli)-conventional molecular ecological approaches, such as PCR-DGGE and qPCR, still remain a very useful complementary tool. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink