Platelet alloimmunization after multiple transfusions: a prospective study of 50 patients
| Autor: | Thérèse Seror, Bertrand Godeau, Najib Duedari, Patricia Fromont, Philippe Bierling |
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| Rok vydání: | 1992 |
| Předmět: |
Adult
Blood Platelets Male Adolescent medicine.drug_class Fluorescent Antibody Technique Monoclonal antibody Antigen Blood product HLA Antigens Isoantibodies Immunopathology Medicine Humans Platelet Blood Transfusion Prospective Studies Child Aged Aged 80 and over biology business.industry Autoantibody Antibodies Monoclonal Hematology Middle Aged Thrombocytopenia Platelet transfusion Immunoglobulin M Immunoglobulin G Immunology biology.protein Female Immunization Antibody business |
| Zdroj: | British journal of haematology. 81(3) |
| ISSN: | 0007-1048 |
| Popis: | Fifty polytransfused patients were prospectively studied to determine the frequency of post-transfusion alloimmunization and its influence on the response to platelet transfusion. Platelet- and HLA-specific antibodies were detected by means of the standard and antiglobulin-augmented lymphocytotoxicity techniques (LCT), the platelet suspension indirect immunofluorescence test (PSIIFT), and monoclonal antibody immobilization of platelet antigens (MAIPA). HLA antibodies were detected in 13 patients (26%) (IgM = 6; IgG = 6; IgM + IgG = 1). The standard LCT was positive in 12 of these 13 patients. Complement-independent HLA antibodies, only detectable in the PSIIFT and the antiglobulin-augmented LCT, were documented in two patients and were associated with poor post-transfusion platelet recovery in the patient who could be evaluated. All the HLA antibodies were detected in the PSIIFT, while only four were detected in the MAIPA. Platelet-specific alloantibodies were found in two patients by means of PSIIFT or MAIPA and may have led to poor post-transfusion platelet recovery in one patient. Platelet autoantibodies were detected in two patients but were not associated with platelet refractoriness. Paraformaldehyde-dependent platelet antibodies were detected in 11 patients but were not associated with platelet refractoriness. |
| Databáze: | OpenAIRE |
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