Comparative structural and functional analysis of photoreceptor neurons of Rho-/- mice reveal increased survival on C57BL/6J in comparison to 129Sv genetic background
| Autor: | Maryanne Donovan, Paul F. Kenna, Shigeki Machida, Thomas G. Cotter, Audrey Hobson, Sophie Kiang, Peter Humphries, Niamh McNally, Paul A. Sieving, Ronald A. Bush, Marian M. Humphries, Jane Farrar |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Aging
Rhodopsin Physiology Cell Survival Congenic Mice Inbred Strains Biology medicine.disease_cause Retina chemistry.chemical_compound Mice Species Specificity Retinitis pigmentosa medicine Electroretinography Animals Outer nuclear layer Genetics Cell Nucleus Mice Knockout Mutation medicine.diagnostic_test Retinal medicine.disease Sensory Systems Cell biology Mice Inbred C57BL medicine.anatomical_structure Phenotype chemistry Nerve Degeneration Retinal Cone Photoreceptor Cells sense organs Erg Photoreceptor Cells Vertebrate |
| Zdroj: | Visual neuroscience. 18(3) |
| ISSN: | 0952-5238 |
| Popis: | To explore the possible influence of defined genetic backgrounds on photoreceptor viability and function in mice carrying a targeted disruption of the rhodopsin gene, the severities of retinopathies in Rho-/- mice on C57BL/6J and 129Sv congenic backgrounds were compared by light microscopy and electroretinography and qualitatively by in situ end labeling of DNA in apoptotic photoreceptor nuclei of retinal sections. Cone photoreceptor viability and function were shown to deteriorate more slowly on the C57BL/6J background in comparison to that of the 129Sv, with significantly greater numbers of outer nuclear layer nuclei in the retinas of C57BL/6J mice at 3 and 4 months of age. Both amplitude and waveform features of the ERG were shown to be remarkably different in the two strains, indicating an approximately 6-fold difference in C57BL/6J Rho-/- mice compared to 129Sv Rho-/- mice at 80 days. Thus, in comparison with the 129Sv strain, genetic modifiers appear to constitute a component of the C57BL/6J background, the expression of which significantly protects cone photoreceptors from apoptotic death in a mutation-induced murine retinopathy. The differences in phenotype revealed in this study are sufficient in principle to provide a basis for comparisons to be made between QTLs in light-induced and mutation-induced systems. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|