Patterning of photosensitive polyimide LB film and its application in the fabrication of biomolecular microphotodiode array
| Autor: | Joon-Kyu Park, Chang-Bum Ko, Jeong-Woo Choi, Se Young Oh |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Materials science
Manufactured Materials Cytochrome Light Photochemistry Biomedical Engineering Biophysics Analytical chemistry Biosensing Techniques Sensitivity and Specificity law.invention Coated Materials Biocompatible law Biomimetic Materials Monolayer Electrochemistry Thin film Miniaturization biology Cytochrome c Electric Conductivity Substrate (chemistry) Cytochromes c Reproducibility of Results Membranes Artificial General Medicine Equipment Design Enzymes Immobilized Equipment Failure Analysis Resins Synthetic Chemical engineering Semiconductors biology.protein Adsorption Scanning tunneling microscope Cyclic voltammetry Microelectrodes Polyimide Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 19(2) |
| ISSN: | 0956-5663 |
| Popis: | Ultra thin film of photosensitive polyimide having benzene and sulfonyloxyimide moieties in the main chain was prepared using a Langmuir-Blodgett (LB) technique, and then micro array pattern of the polyimide LB film on a gold substrate was obtained by deep UV lithographic technique. In order to array cytochrome c molecules along the micro-patterned gold substrate, the well-characterized monolayer of cytochrome c was immobilized with a mixed monolayer of 11-mercaptoundecanoic acid (11-MUDA) and decanethiol. The redox activity and electron transfer between cytochrome c molecular center and gold electrode interface for the self-assembled cytochrome c monolayer were investigated by cyclic voltammetry measurement. Biomolecular photodiode consisting of cytochrome c and green fluorescent protein (GFP) onto the patterned gold substrate was fabricated by self-assembly process. The integration and morphology of cytochrome c and GFP were studied from the measurements of atomic force microscopy (AFM) and fluorescence emission. Especially, current-voltage characteristics of the protein multilayers were investigated by scanning tunneling microscopy (STM) and its application in biomolecular photodiode was also examined. |
| Databáze: | OpenAIRE |
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