Identification of three homologous latex-clearing protein (lcp) genes from the genome of Streptomyces sp. strain CFMR 7
| Autor: | Su Yean Ong, Jayaram Nanthini, Kumar Sudesh |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Latex In silico 030106 microbiology complex mixtures Streptomyces Genome 03 medical and health sciences Bacterial Proteins Genetics Gene Whole genome sequencing biology Strain (chemistry) Gordonia polyisoprenivorans technology industry and agriculture General Medicine biology.organism_classification body regions 030104 developmental biology Biochemistry Rubber Oxidoreductases Genome Bacterial Bacteria |
| Zdroj: | Gene. 628:146-155 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2017.07.039 |
| Popis: | Rubber materials have greatly contributed to human civilization. However, being a polymeric material does not decompose easily, it has caused huge environmental problems. On the other hand, only few bacteria are known to degrade rubber, with studies pertaining them being intensively focusing on the mechanism involved in microbial rubber degradation. The Streptomyces sp. strain CFMR 7, which was previously confirmed to possess rubber-degrading ability, was subjected to whole genome sequencing using the single molecule sequencing technology of the PacBio® RS II system. The genome was further analyzed and compared with previously reported rubber-degrading bacteria in order to identify the potential genes involved in rubber degradation. This led to the interesting discovery of three homologues of latex-clearing protein (Lcp) on the chromosome of this strain, which are probably responsible for rubber degrading activities. Genes encoding oxidoreductase α-subunit (oxiA) and oxidoreductase β-subunit (oxiB) were also found downstream of two lcp genes which are located adjacent to each other. In silico analysis reveals genes that have been identified to be involved in the microbial degradation of rubber in the Streptomyces sp. strain CFMR 7. This is the first whole genome sequence of a clear-zone-forming natural rubber- degrading Streptomyces sp., which harbours three Lcp homologous genes with the presence of oxiA and oxiB genes compared to the previously reported Gordonia polyisoprenivorans strain VH2 (with two Lcp homologous genes) and Nocardia nova SH22a (with only one Lcp gene). |
| Databáze: | OpenAIRE |
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