| Autor: |
Sheng Yang Lee, Yen Hua Huang, Yen Chuang Lin, Jen Chang Yang, Shu Li Lin, Haw Ming Huang |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Journal of Dental Sciences, Vol 9, Iss 3, Pp 244-248 (2014) |
| ISSN: |
1991-7902 |
| Popis: |
Background/purpose: The aim of this study was to investigate whether storage of extracted teeth in transportation solution for 24 hours has a negative effect on the cell density and mechanical properties of thawed pulp tissue extracted from frozen intact teeth. Material and methods: Human premolars were kept in transportation solution for 0 hours or 24 hours. For each transportation time, the tested teeth were divided into two experimental groups: teeth that had been frozen in a magnetic programmed freezer (PF, n = 5) and in a traditional freezer at −20°C (TF, n = 5). The tested teeth were then stored at −150°C for 7 days. After thawing, the extracted pulp was subjected to a histological examination and mechanical testing. Results: Storage in transportation solution for 24 hours had no significant negative effects on the histological or mechanical properties of the pulp tissue extracted from the cryopreserved intact teeth. The elastic modulus of pulp from the teeth that had been frozen at −20°C was significantly higher (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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