VOC fingerprints: metabolomic signatures of biothreat agents with and without antibiotic resistance
Autor: | Amanda Haymond, Hafsa A. Abdirahman, Allyson Dailey, Farhang Alem, Jessica Saha, Fatima Zaidi, Robin D. Couch, Ramin M. Hakami |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Yersinia pestis Pneumonia Viral 030106 microbiology lcsh:Medicine Article Disease Outbreaks Microbiology Betacoronavirus Mice 03 medical and health sciences Burkholderia mallei Antibiotic resistance Kanamycin Metabolome Animals Metabolomics lcsh:Science Francisella tularensis Pandemics Tularemia Solid Phase Microextraction Mice Inbred BALB C Volatile Organic Compounds Multidisciplinary biology SARS-CoV-2 lcsh:R COVID-19 Outbreak Drug Resistance Microbial biology.organism_classification 030104 developmental biology Microbial genetics lcsh:Q Female Coronavirus Infections Anti-Infective Agents |
Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) |
ISSN: | 2045-2322 |
Popis: | Category A and B biothreat agents are deemed to be of great concern by the US Centers for Disease Control and Prevention (CDC) and include the bacteria Francisella tularensis, Yersinia pestis, Burkholderia mallei, and Brucella species. Underscored by the impact of the 2020 SARS-CoV-2 outbreak, 2016 Zika pandemic, 2014 Ebola outbreak, 2001 anthrax letter attacks, and 1984 Rajneeshee Salmonella attacks, the threat of future epidemics/pandemics and/or terrorist/criminal use of pathogenic organisms warrants continued exploration and development of both classic and alternative methods of detecting biothreat agents. Volatile organic compounds (VOCs) comprise a large and highly diverse group of carbon-based molecules, generally related by their volatility at ambient temperature. Recently, the diagnostic potential of VOCs has been realized, as correlations between the microbial VOC metabolome and specific bacterial pathogens have been identified. Herein, we describe the use of microbial VOC profiles as fingerprints for the identification of biothreat-relevant microbes, and for differentiating between a kanamycin susceptible and resistant strain. Additionally, we demonstrate microbial VOC profiling using a rapid-throughput VOC metabolomics method we refer to as ‘simultaneous multifiber headspace solid-phase microextraction’ (simulti-hSPME). Finally, through VOC analysis, we illustrate a rapid non-invasive approach to the diagnosis of BALB/c mice infected with either F. tularensis SCHU S4 or Y. pestis CO92. |
Databáze: | OpenAIRE |
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