Nitric oxide synthase inhibitor suppresses the ototoxic side effect of cisplatin in guinea pigs
| Autor: | Wilhelm Bloch, Alexander Hess, Ken-ichi Watanabe, Olaf Michel |
|---|---|
| Přispěvatelé: | Vriendenkring VUB |
| Rok vydání: | 2000 |
| Předmět: |
Cancer Research
NOS inhibitor Side effect Guinea Pigs Nitric Oxide Synthase Type II inner ear damage Antineoplastic Agents Pharmacology Threshold shift L-NAME Ototoxicity otorhinolaryngologic diseases medicine Evoked Potentials Auditory Brain Stem Animals Pharmacology (medical) Inner ear Enzyme Inhibitors Hearing Disorders Cochlea hearing loss Cisplatin biology Chemistry Auditory Threshold medicine.disease Immunohistochemistry Cochlea Nitric Oxide iNOS Nitric oxide synthase ototoxicity medicine.anatomical_structure NG-Nitroarginine Methyl Ester Oncology Cisplatin/adverse effects biology.protein Drug Therapy Combination sense organs Nitric Oxide Synthase medicine.drug |
| Zdroj: | Anti-cancer drugs. 11(5) |
| ISSN: | 0959-4973 |
| Popis: | Cisplatin is known to cause inner ear damage (ototoxicity). The role of inducible nitric oxide synthase (iNOS) in the cochlea of guinea pigs after injections of cisplatin or a combination of cisplatin and NOS inhibitor (NG-nitro-L-arginine methyl ester, L-NAME) i.p. was examined electro- and immunohistochemically. The auditory brain stem responses (ABR) were measured prior to injection and 3 days after the injection. Three days after injection, the cochleas were examined immunohistochemically for iNOS. We found that iNOS was expressed in the cisplatin- and L-NAME/cisplatin-treated cochlea. The threshold shift of ABR was significant in the cisplatin group, whereas it was decreased in the L-NAME/cisplatin group. iNOS catalyzed high NO levels lead to inner ear dysfunction. Our results indicate that iNOS mediates the ototoxicity of cisplatin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|