Pyridine: a Denaturant or Stabilizer of Spherical Nucleic Acids?
| Autor: | Ju Hwan Oh, Jae Seung Lee, Yoon Hyuck Kim, Abigail K. R. Lytton-Jean |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Dna duplex Pyridines Stereochemistry Melting temperature Metal Nanoparticles Nucleic Acid Denaturation PH increase 01 natural sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Pyridine Transition Temperature Neutral ph 010405 organic chemistry Nucleic Acid Hybridization DNA Hydrogen-Ion Concentration 0104 chemical sciences Crystallography 030104 developmental biology chemistry Duplex (building) Nucleic acid Gold |
| Zdroj: | Analytical Chemistry. 89:4581-4586 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.7b00005 |
| Popis: | The spotlighted dual functions of pyridine as a denaturant and as a stabilizer for duplex DNA are thoroughly investigated using spherical nucleic acids (SNAs). At neutral pH, pyridine destabilizes the duplex interconnects of assembled SNAs, resulting in a gradual decrease in their melting temperature (Tm) as a function of the pyridine concentration. This result is in good agreement with the conventional role of pyridine as a powerful denaturant for free duplex DNA. On the contrary, the addition of pyridine dramatically increases the Tm of hybridized SNAs under acidic conditions, which could be a striking result of pyridine’s stabilizing effect for DNA duplex as previously suggested on the basis of the pyridine–nucleobase interactions. After comprehensive and quantitative investigation based on the analysis of the sharp melting transitions of SNAs, however, we report that, in fact, the pH increase induced by pyridine is also an essential parameter accounting for pyridine’s DNA-stabilizing effects under aci... |
| Databáze: | OpenAIRE |
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