Monoclonal antibodies against Haemophilus lipopolysaccharides: clone DP8 specific for Haemophilus ducreyi and clone DH24 binding to lacto-N-neotetraose
| ISSN: | 1098-5522 0019-9567 |
|---|---|
| DOI: | 10.1128/iai.63.7.2665-2673.1995 |
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e162b8dff777c541ce049e8ea66d543 https://doi.org/10.1128/iai.63.7.2665-2673.1995 |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.doi.dedup.....0e162b8dff777c541ce049e8ea66d543 |
| Autor: | A. A. Lindberg, H J Ahmed, Silvia Borrelli, J. Jonasson, Erwin Ludo Roggen, Peter Piot, D Hendriksen, Per-Erik Jansson |
| Rok vydání: | 1995 |
| Předmět: |
Lipopolysaccharides
Glycoconjugate medicine.drug_class Molecular Sequence Data Immunology Neuraminidase Oligosaccharides urologic and male genital diseases Monoclonal antibody medicine.disease_cause Binding Competitive Microbiology Epitope Haemophilus influenzae Haemophilus ducreyi Lipid A Mice Species Specificity Antibody Specificity Haemophilus medicine Animals chemistry.chemical_classification Antigens Bacterial Mice Inbred BALB C biology Antibodies Monoclonal biology.organism_classification Antibodies Bacterial Infectious Diseases Epitope mapping Carbohydrate Sequence chemistry Parasitology Glycoconjugates Epitope Mapping Research Article |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.63.7.2665-2673.1995 |
| Popis: | Mouse monoclonal antibodies (MAbs) DP8 [immunoglobulin G1(kappa)] and DH24 [immunoglobulin M(kappa)], which are specific for Haemophilus ducreyi lipopolysaccharide (LPS), were generated by fusing mouse myeloma NS0 cells with spleen cells of BALB/c mice immunized with a total membrane preparation of H. ducreyi. MAb DP8 reacted in whole-cell enzyme immunoassay (EIA) and colony dot immunoblotting with all 50 strains of H. ducreyi but not with any other bacteria tested, which suggests an exposed and species-specific epitope on the H. ducreyi cell surface. This conclusion was supported by the finding that DP8 bound to all six H. ducreyi LPSs tested but not to any of the Haemophilus influenzae or enterobacterial LPSs or synthetic glycoconjugates. The MAb DH24 bound to 43 of 50 strains of H. ducreyi and to few strains of H. influenzae, Neisseria gonorrhoeae, and Neisseria meningitidis, as evaluated by whole-cell EIA and colony dot immunoblotting. The MAb DH24 reacted with five of the six H. ducreyi LPSs tested and with the lacto-N-neotetraose (Gal beta 1-->4GlcNAc beta 1-->3Gal beta 1-->4Glc) series of synthetic glycoconjugates, as determined by EIA. By using polysaccharides obtained after both mild acidic hydrolysis and strong alkali treatment and dephosphorylated samples as inhibitors of the MAbs binding to H. ducreyi LPS antigens, it could be shown that phosphate groups were essential for the binding of DP8 to LPS but that they did not affect antigenic recognition by DH24. None of the MAbs bound to isolated lipid A, but aggregation caused by the fatty acids of lipid A was essential for epitope recognition. |
| Databáze: | OpenAIRE |
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