Usefulness of detection of clarithromycin-resistant Helicobacter pylori from fecal specimens for young adults treated with eradication therapy
| Autor: | Shinji Fujieda, Mitsuhide Goto, Shigeru Kamiya, Masumi Okuda, Katsuhiro Mabe, Takako Osaki, Mototsugu Kato, Cynthia Zaman, Hideo Yonezawa, Kenji Amagai, Wataru Shibata |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult DNA Bacterial Male medicine.medical_specialty Adolescent medicine.drug_class Biopsy 030106 microbiology Antibiotics Microbial Sensitivity Tests Gene mutation Gastroenterology Polymerase Chain Reaction law.invention Helicobacter Infections 03 medical and health sciences Feces Young Adult 0302 clinical medicine Antibiotic resistance law Internal medicine Clarithromycin Drug Resistance Bacterial medicine Humans Polymerase chain reaction biology Helicobacter pylori business.industry General Medicine Gold standard (test) Sequence Analysis DNA biology.organism_classification Anti-Bacterial Agents Infectious Diseases Gastric Mucosa Mutation 030211 gastroenterology & hepatology Female business medicine.drug |
| Zdroj: | Helicobacter. 22(5) |
| ISSN: | 1523-5378 |
| Popis: | Background To prevent Helicobacter pylori infection in the younger generation, it is necessary to investigate the prevalence of antibiotic-resistant H. pylori. Objective The aim of this study was to evaluate the method of PCR-based sequencing to detect clarithromycin (CAM) resistance-associated mutations using fecal samples as a noninvasive method. Methods DNA extracted from fecal specimens and isolates from gastric biopsy specimens were collected from patients with H. pylori infection. Antibiotic resistance to CAM was analyzed by molecular and culture methods. The detection rates of CAM resistance-associated mutations (A2142C or A2143G) were compared before and after eradication therapy. Results With CAM resistance of H. pylori evaluated by antibiotic susceptibility test as a gold standard, the sensitivity and the specificity of gene mutation detection from fecal DNA were 80% and 84.8%, respectively. In contrast, using DNA of isolated strains, the sensitivity and the specificity were 80% and 100%. Of the seven cases in which eradication was unsuccessful by triple therapy including CAM, CAM-resistant H. pylori, and resistance-associated mutations were detected in three cases, CAM-resistant H. pylori without the mutation was detected in two patients, and resistance-associated mutation was only detected in one patient. Conclusion PCR-based sequencing to detect CAM resistance-associated mutations using isolates or fecal samples was useful for finding antibiotic-resistant H. pylori infection. Although the specificity of the detection from fecal samples compared with antibiotic susceptibility testing was lower than that from isolates, this fecal detection method is suitable especially for asymptomatic subjects including children. Further improvement is needed before clinical application. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|