Essential role of phosphatidylinositol 3-kinase in insulin-induced activation and phosphorylation of the cGMP-inhibited cAMP phosphodiesterase in rat adipocytes studies using the selective inhibitor wortmannin
| Autor: | Tova Rahn, Martin Ridderstråle, Vincent C. Manganiello, Per Belfrage, Hans Tornqvist, Gudrun Fredrikson, Eva Degerman |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Male
medicine.medical_specialty Inhibitor medicine.medical_treatment Lipolysis Phosphodiesterase 3 Biophysics Protein Serine-Threonine Kinases Biochemistry Wortmannin Rats Sprague-Dawley chemistry.chemical_compound Phosphatidylinositol 3-Kinases Structural Biology Internal medicine Adipocyte health services administration mental disorders Genetics medicine Adipocytes Animals Insulin Phosphatidylinositol Phosphorylation Molecular Biology Cyclic GMP cGMP-inhibited cAMP phosphodiesterase Chemistry Kinase Phosphodiesterase Cell Biology humanities Cell biology Rats Androstadienes Enzyme Activation Phosphotransferases (Alcohol Group Acceptor) enzymes and coenzymes (carbohydrates) Endocrinology 3' 5'-Cyclic-AMP Phosphodiesterases sense organs Phosphatidylinositol 3-kinase |
| Zdroj: | FEBS Letters. (2-3):314-318 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(94)00797-7 |
| Popis: | Incubation of rat adipocytes with wortmannin, a potent and selective phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor, completely blocked the antilipolytic action of insulin (IC50≈ 100 nM), the insulin-induced activation and phosphorylation of cGMP-inhibited cAMP phosphodiesterase (cGI-PDE) as well as the activation of the insulin-stimulated cGI-PDE kinase (IC50≈ 10–30 nM). No direct effects of the inhibitor on the insulin-stimulated cGI-PDE kinase, the cGI-PDE and the hormone-sensitive lipase were observed. These data suggest that activation of PI 3-kinase upstream of the insulin-stimulated cGI-PDE kinase in the antilipolytic insulin signalchain has an essential role for insulin-induced cGI-PDE activation/ phosphorylation and anti-lipolysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|