Sustained Levels of FGF2 Maintain Undifferentiated Stem Cell Cultures with Biweekly Feeding
| Autor: | Ravi S. Kane, Steven Lotz, Jeffrey H. Stern, Barbara Corneo, Sally Temple, Faizzan Ahmad, Susan K. Goderie, Sheila Le, Nicolas Tokas, Sarah E. Hirsch, Christopher A. Fasano, Akhilesh Banerjee |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
KOSR
MAP Kinase Signaling System Cellular differentiation Induced Pluripotent Stem Cells Cell Culture Techniques lcsh:Medicine Biology Stem cell marker Biochemistry Mice Neural Stem Cells Polylactic Acid-Polyglycolic Acid Copolymer Growth Factors Molecular Cell Biology Animals Humans Lactic Acid Stem Cell Niche lcsh:Science Induced pluripotent stem cell Embryonic Stem Cells Cells Cultured Multidisciplinary integumentary system Stem Cells lcsh:R Proteins Cell Differentiation Cells Immobilized Embryonic stem cell Neural stem cell Microspheres Cell biology Culture Media Enzyme Activation Cell culture embryonic structures lcsh:Q Fibroblast Growth Factor 2 Stem cell Cellular Types Mitogen-Activated Protein Kinases Polyglycolic Acid Research Article Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 2, p e56289 (2013) |
| ISSN: | 1932-6203 |
| Popis: | An essential aspect of stem cell culture is the successful maintenance of the undifferentiated state. Many types of stem cells are FGF2 dependent, and pluripotent stem cells are maintained by replacing FGF2-containing media daily, while tissue-specific stem cells are typically fed every 3rd day. Frequent feeding, however, results in significant variation in growth factor levels due to FGF2 instability, which limits effective maintenance due to spontaneous differentiation. We report that stabilization of FGF2 levels using controlled release PLGA microspheres improves expression of stem cell markers, increases stem cell numbers and decreases spontaneous differentiation. The controlled release FGF2 additive reduces the frequency of media changes needed to maintain stem cell cultures, so that human embryonic stem cells and induced pluripotent stem cells can be maintained successfully with biweekly feedings. |
| Databáze: | OpenAIRE |
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