Growth hormone secretagogue receptor in dopamine neurons controls appetitive and consummatory behaviors towards high-fat diet in ad-libitum fed mice

Autor: Mario Perello, Daniela Cassano, Pablo Nicolás de Francesco, Maria Florencia Andreoli, María Paula Cornejo, Mirta Reynaldo, Guadalupe García Romero, Franco Barrile, Julieta Paola Aguggia
Rok vydání: 2020
Předmět:
Male
Endocrinology
Diabetes and Metabolism
Growth hormone secretagogue receptor
DOPAMINE
Eating
Mice
0302 clinical medicine
Endocrinology
APPETITE
high-fat
Receptor
Receptors
Ghrelin
media_common
Behavior
Animal
digestive
oral
and skin physiology
purl.org/becyt/ford/3.1 [https]
Psychiatry and Mental health
appetite
ghrelin
HIGH-FAT
purl.org/becyt/ford/3 [https]
Ghrelin
dopamine
Consummatory Behavior
medicine.drug
medicine.medical_specialty
MESOCORTICOLIMBIC PATHWAY
Medicina
media_common.quotation_subject
mesocorticolimbic pathway
Cre recombinase
Mice
Transgenic
Biology
Diet
High-Fat
03 medical and health sciences
Food Preferences
Dopamine
Orexigenic
Internal medicine
High fat
medicine
Animals
GHRELIN
Biological Psychiatry
Endocrine and Autonomic Systems
Appetite Regulation
Dopaminergic Neurons
Appetite
Feeding Behavior
030227 psychiatry
Mice
Inbred C57BL
GHSR
030217 neurology & neurosurgery
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 1873-3360
Popis: Growth hormone secretagogue receptor (GHSR), the receptor for ghrelin, is expressed in key brain nuclei that regulate food intake. The dopamine (DA) pathways have long been recognized to play key roles mediating GHSR effects on feeding behaviors. Here, we aimed to determine the role of GHSR in DA neurons controlling appetitive and consummatory behaviors towards high fat (HF) diet. For this purpose, we crossed reactivable GHSR-deficient mice with DA transporter (DAT)-Cre mice, which express Cre recombinase under the DAT promoter that is active exclusively in DA neurons, to generate mice with GHSR expression limited to DA neurons (DAT-GHSR mice). We found that DAT-GHSR mice show an increase of c-Fos levels in brain areas containing DA neurons after ghrelin treatment, in a similar fashion as seen in wild-type mice; however, they did not increase food intake or locomotor activity in response to systemically- or centrally-administered ghrelin. In addition, we found that satiated DAT-GHSR mice displayed both anticipatory activity to scheduled HF diet exposure and HF intake in a binge-like eating protocol similar to those in wild-type mice, whereas GHSR-deficient mice displayed impaired responses. We conclude that GHSR expression in DA neurons is sufficient to both mediate increased anticipatory activity to a scheduled HF diet exposure and fully orchestrate binge-like HF intake, but it is insufficient to restore the acute orexigenic or locomotor effects of ghrelin treatment. Thus, GHSR in DA neurons affects appetitive and consummatory behaviors towards HF diet that take place in the absence of caloric needs.
Instituto Multidisciplinario de Biología Celular
Databáze: OpenAIRE
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